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DatoValore
TitlePhytochelatins in the diatom Phaeodactylum tricornutum Bohlin: an evaluation of their use as biomarkers of metal exposure in marine waters.
AbstractThe feasibility of a bioassay based on the synthesis of phytochelatins to assess metal pollution in aquatic environments was evaluated by using the marine diatom Phaeodactylum tricornutum. Short-term incubation experiments carried out in EDTA-buffered artificial seawater showed increasing cellular phytochelatin concentration with increasing free cadmium, lead or copper in the medium, indicating that phytochelatins behave as a biomarker of exposure to the bioavailable metal fraction. A linear dose-response relationship between metal exposure and phytochelatin synthesis was found in natural seawater samples enriched with known amounts of heavy metals. Phytochelatin induction tests carried out on polluted seawater samples showed an enhanced response compared to that obtained in unpolluted seawater. This finding was found to be consistent mainly with a copper contamination.
SourceBulletin of environmental contamination and toxicology 81, pp. 236–241
JournalBulletin of environmental contamination and toxicology
EditorSpringer Science and Business Media, New York, NY, Stati Uniti d'America
Year2008
TypeArticolo in rivista
DOI10.1007/s00128-008-9472-z
AuthorsMorelli E.; Fantozzi L.
Text9699 2008 10.1007/s00128 008 9472 z ISI Web of Science WOS 000258275700003 Phytochelatins in the diatom Phaeodactylum tricornutum Bohlin an evaluation of their use as biomarkers of metal exposure in marine waters. Morelli E.; Fantozzi L. E. Morelli, L. Fantozzi Istituto di Biofisica CNR , Area della Ricerca di Pisa, Via Moruzzi 1, 56124 Pisa, Italy The feasibility of a bioassay based on the synthesis of phytochelatins to assess metal pollution in aquatic environments was evaluated by using the marine diatom Phaeodactylum tricornutum. Short term incubation experiments carried out in EDTA buffered artificial seawater showed increasing cellular phytochelatin concentration with increasing free cadmium, lead or copper in the medium, indicating that phytochelatins behave as a biomarker of exposure to the bioavailable metal fraction. A linear dose response relationship between metal exposure and phytochelatin synthesis was found in natural seawater samples enriched with known amounts of heavy metals. Phytochelatin induction tests carried out on polluted seawater samples showed an enhanced response compared to that obtained in unpolluted seawater. This finding was found to be consistent mainly with a copper contamination. 81 Articolo pubblicato BECT_2008.pdf Articolo in rivista Springer Science and Business Media 0007 4861 Bulletin of environmental contamination and toxicology Bulletin of environmental contamination and toxicology Bull. environ. contam. toxicol. Bulletin of environmental contamination and toxicology. Bulletin of environmental contamination and toxicology Print laura.fantozzi FANTOZZI LAURA elisabetta.morelli MORELLI ELISABETTA TA.P05.015.002 Meccanismi di risposta cellulare a contaminanti ambientali