Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbial ecology approach for evaluating the effects of contaminants on soil and water ecosystems |
| Abstract | Traditional parameters used for the Environmental Risk Assessment (ERA) of environmental contaminants are principally chemiodynamic and physico-chemical properties. Furthermore ecotoxicological effects on organisms in water and soil are assessed with standard acute and chronic ecotoxicologic tests on freshwater, marine and soil organisms. Microbial communities are valuable indicators of the occurrence of disturbances due to exogenous physico-chemical stressors. The assessment of variations in microbial community structure is of basic importance in order to evaluate the impact of an environmental stressor. Complex microbial communities may serve as ideal and ecologically relevant toxicity indicators. A number of microbiologically driven processes has been proposed to evaluate the effects of xenobiotics on ecosystems. Proteins, genes, metabolites, or lipids that, when expressed, present a pattern of molecular change in an organism in response to a specific environmental stressor, can be defined as environmental biomarkers. The number of techniques to study microbial communities has increased exponentially over the last 20 years and the advent of culture-independent methods, such as molecular biological techniques, has changed the view of microbial diversity. Among these techniques epifluorescence microscopy ones, such as direct count of bacterial abundance (DAPI count), vitality (Live/Dead cell viability assay), and Fluorescence In Situ Hybridization (FISH) have been showing effective tools for studying specific microbial populations in soil and water ecosystems. We show how the applications of these techniques to contaminated soil and water ecosystems allow to highlight the presence of particular bacterial groups involved in chemical degradation. |
| Source | SETAC Europe 21st Annual Meeting - Ecosystem Protection in a Sustainable World: A Challenge for Science and Regulation, Milano, 15-19 May 2011 |
| Year | 2011 |
| Type | Contributo in atti di convegno |
| Authors | Grenni P, Barra Caracciolo A |
| Text | 89818 2011 Microbial ecology approach for evaluating the effects of contaminants on soil and water ecosystems Grenni P, Barra Caracciolo A Grenni P, Barra Caracciolo A IRSA CNR Traditional parameters used for the Environmental Risk Assessment ERA of environmental contaminants are principally chemiodynamic and physico chemical properties. Furthermore ecotoxicological effects on organisms in water and soil are assessed with standard acute and chronic ecotoxicologic tests on freshwater, marine and soil organisms. Microbial communities are valuable indicators of the occurrence of disturbances due to exogenous physico chemical stressors. The assessment of variations in microbial community structure is of basic importance in order to evaluate the impact of an environmental stressor. Complex microbial communities may serve as ideal and ecologically relevant toxicity indicators. A number of microbiologically driven processes has been proposed to evaluate the effects of xenobiotics on ecosystems. Proteins, genes, metabolites, or lipids that, when expressed, present a pattern of molecular change in an organism in response to a specific environmental stressor, can be defined as environmental biomarkers. The number of techniques to study microbial communities has increased exponentially over the last 20 years and the advent of culture independent methods, such as molecular biological techniques, has changed the view of microbial diversity. Among these techniques epifluorescence microscopy ones, such as direct count of bacterial abundance DAPI count , vitality Live/Dead cell viability assay , and Fluorescence In Situ Hybridization FISH have been showing effective tools for studying specific microbial populations in soil and water ecosystems. We show how the applications of these techniques to contaminated soil and water ecosystems allow to highlight the presence of particular bacterial groups involved in chemical degradation. Ecosystem Protection in a Sustainable World A Challenge for Science and Regulation SETAC Europe http //c.ymcdn.com/sites/www.setac.org/resource/resmgr/Abstract_Books/SE abstract book 2011.pdf SETAC Europe 21st Annual Meeting Ecosystem Protection in a Sustainable World A Challenge for Science and Regulation Milano 15 19 May 2011 Internazionale Contributo Abstract Book SE_abstract_book_2011 0.pdf Contributo in atti di convegno anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |