| Abstract | BTEX, MTBE, aliphatic and aromatic hydrocarbons are common groundwater contaminants, in areas where petrochemical activities have been carried out. A long term experimentation (18 months) has led to high rate MTBE removal bacterial cultures, developed in aerobic conditions without external carbon source addition, trough first, second and third generation aerobic microcosms, originally inoculated with the contaminated area subsoil. The Area is inside one of the 56 National Interest Site, contaminated for long time with BTEX and MTBE. MTBE degradation kinetic, in the third generation microcosms is 20 mg MTBE g-1 VSS h-1
(12-30 mg/L MTBE initial concentrations), with residual MTBE lower 10 ?g/L, that is the maximum limit for groundwater to be remediated according the Italian legislation. FISH was applied to reveal the microbial composition of the enrichment developed: the main biomass component is a large pale coccus (75% of DAPI positive micro-organisms). An in situ biological aerobic process, with nutrients and air addition can find application for the bioremediation of the site, stimulating the development of natural micro-organisms present at the contaminated Site. |
| Text | 89711 2008 HIGH MTBE REMOVAL FROM A MICROBIAL CONSORTIUM ENRICHED AT AN ITALIAN NATIONAL INTEREST SITE VOLPE A., DEL MORO G., ROSSETTI S., TANDOI V. VOLPE A. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Sezione di Bari, Via Francesco De Blasio, 5, 70123 Bari, Italy DEL MORO G. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Sezione di Bari, Via Francesco De Blasio, 5, 70123 Bari, Italy ROSSETTI S. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Area della Ricerca Roma 1 Montelibretti, Via Salaria km. 29.300, 00015 Monterotondo RM , Italy TANDOI V. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Area della Ricerca Roma 1 Montelibretti, Via Salaria km. 29.300, 00015 Monterotondo RM , Italy BTEX, MTBE, aliphatic and aromatic hydrocarbons are common groundwater contaminants, in areas where petrochemical activities have been carried out. A long term experimentation 18 months has led to high rate MTBE removal bacterial cultures, developed in aerobic conditions without external carbon source addition, trough first, second and third generation aerobic microcosms, originally inoculated with the contaminated area subsoil. The Area is inside one of the 56 National Interest Site, contaminated for long time with BTEX and MTBE. MTBE degradation kinetic, in the third generation microcosms is 20 mg MTBE g 1 VSS h 1 12 30 mg/L MTBE initial concentrations , with residual MTBE lower 10 g/L, that is the maximum limit for groundwater to be remediated according the Italian legislation. FISH was applied to reveal the microbial composition of the enrichment developed the main biomass component is a large pale coccus 75% of DAPI positive micro organisms . An in situ biological aerobic process, with nutrients and air addition can find application for the bioremediation of the site, stimulating the development of natural micro organisms present at the contaminated Site. ConSoil 2008 Proceedings 978 3 00 024598 5 http //consoil.olanis.de/consoil_previous/consoil_2008/index.php Gli atti del convegno sono disponibili su CD distribuito ai partecipanti. CONSOIL 2008, THE 10TH INTERNATIONAL UFZ Deltares/TNO CONFERENCE ON SOIL WATER SYSTEMS MILANO 3 6 Giugno 2008 Internazionale Contributo HIGH MTBE REMOVAL FROM A MICROBIAL CONSORTIUM ENRICHED AT AN ITALIAN NATIONAL INTEREST SITE Consoil_2008_Full_Paper.pdf Contributo in atti di convegno guido.delmoro DEL MORO GUIDO simona.rossetti ROSSETTI SIMONA angela.volpe VOLPE ANGELA valter.tandoi TANDOI VALTER TA.P07.014.005 Risanamento di acque di falda |
