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DatoValore
TitleTERBUTHYLAZINE DEGRADATION IN A CONTAMINATED AQUIFER: STUDY OF THE HOMEOSTATIC CAPACITY OF THE AUTOCHTONOUS BACTERIAL COMMUNITY
AbstractThe ability of groundwater to recover from pesticide contamination is primarily dependent on the presence of a microbial community able to degrade them. Neverthless, the study of bacterial population living in groundwater is quite scarce because it is strictly dependent on methods able to identify and characterize their community structure and functioning. In the present work we apply the Fluorescence In situ Hybridization (FISH) method to groundwater samples from a phreatic aquifer, located in the centre of Italy, in order to identify the autochtonous bacterial community. The use of 16S rRNA-targeted oligonucleotide probes, specific for the main phylogenetic levels (Archaea, Eubacteria, Alpha- Beta- Gamma-subdivision of Proteobacteria, Plantomycetes, and Gram-positive bacteria with high a DNA G+C content), and a DAPI stain made it possible to determine the structure of the bacterial community and its response to the addition of the herbicide terbuthylazine in groundwater microcosms treated with it. The biodegradation of the herbicide and the formation of its main metabolites was compared to sterile groundwater samples. The results show that, although at a lower rate compared to soil ecosystems, the groundwater bacterial community has a capability of degrading the herbicide.
SourceXIII SYMPOSIUM PESTICIDE CHEMISTRY-ENVIRONMENTAL FATE AND ECOLOGICAL EFFECTS OF PESTICIDES, PIACENZA, 3-6 Settembre, 2007
Year2007
TypeContributo in atti di convegno
AuthorsBARRA CARACCIOLO A, GRENNI P, SACCÁ ML, FALCONI F, CICCOLI R
Text89492 2007 TERBUTHYLAZINE DEGRADATION IN A CONTAMINATED AQUIFER STUDY OF THE HOMEOSTATIC CAPACITY OF THE AUTOCHTONOUS BACTERIAL COMMUNITY BARRA CARACCIOLO A, GRENNI P, SACCÁ ML, FALCONI F, CICCOLI R BARRA CARACCIOLO A, GRENNI P, FALCONI F IRSA CNR CICCOLI R. ENEA Casaccia, Rome Italy The ability of groundwater to recover from pesticide contamination is primarily dependent on the presence of a microbial community able to degrade them. Neverthless, the study of bacterial population living in groundwater is quite scarce because it is strictly dependent on methods able to identify and characterize their community structure and functioning. In the present work we apply the Fluorescence In situ Hybridization FISH method to groundwater samples from a phreatic aquifer, located in the centre of Italy, in order to identify the autochtonous bacterial community. The use of 16S rRNA targeted oligonucleotide probes, specific for the main phylogenetic levels Archaea, Eubacteria, Alpha Beta Gamma subdivision of Proteobacteria, Plantomycetes, and Gram positive bacteria with high a DNA G C content , and a DAPI stain made it possible to determine the structure of the bacterial community and its response to the addition of the herbicide terbuthylazine in groundwater microcosms treated with it. The biodegradation of the herbicide and the formation of its main metabolites was compared to sterile groundwater samples. The results show that, although at a lower rate compared to soil ecosystems, the groundwater bacterial community has a capability of degrading the herbicide. ENVIRONMENTAL FATE AND ECOLOGICAL EFFECTS OF PESTICIDES Del Re AAM, Capri E, Fragoulis G, Trevisan M 978 88 7830 473 4 http //istituti.unicatt.it/chimica_agraria_e_ambientale_2211.html XIII SYMPOSIUM PESTICIDE CHEMISTRY ENVIRONMENTAL FATE AND ECOLOGICAL EFFECTS OF PESTICIDES PIACENZA 3 6 Settembre, 2007 Internazionale Contributo Articolo pubblicato TBA_Barra.pdf Contributo in atti di convegno francesca.falconi FALCONI FRANCESCA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.007 Strumenti di mitigazione dello stress quali quantitativo per i sistemi idrici