Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Structure-biodegradation correlation of polyphenols for Thauera aromatica K172 in anaerobic conditions |
| Abstract | The anaerobic degradation of phenolic compounds depends greatly on their molecular structure. We have undertaken a systematic study on several phenol derivatives which are commonly found in industrial waters with the aim of investigating the structure-biodegradation relationship for bacteria in anaerobic conditions. The Thauera aromatica strain was used; this bacterium is able to use phenol as a sole carbon source in liquid cultures in the absence of molecular oxygen. Nitrate is the electron sink with complete reduction to N2. A systematic study has demonstrated the ability of this bacterial strain to use 3,4-dihydroxybenzoic acid, 4-hydroxycinnamic acid (p-coumaric acid), 4-hydroxy-3-methoxycinnamic acid (ferulic acid), 3,4-dimethoxybenzoic acid (veratric acid), and 3,4,5-trihydroxybenzoic acid (gallic acid) as substrate. The matching of the depletion of the concentration of such compounds with the increment in bacterial growth in the cultures demonstrates that the substrates are used as the sole carbon source. Also, a correlation between the rate of growth of bacteria and nitrite concentration has been observed. The progressive reduction in nitrate into nitrite and N2 is monitored through analyses. The protein pattern extracted from bacteria grown on methylated substrates is now being compared with that of bacteria grown on non-methylated polyphenols that are structurally analogous in order to identify specific enzymes for demethylation processes. |
| Source | Chemistry in ecology (Print) 22 (1), pp. S133–S143 |
| Keywords | Polyphenols; Anaerobic metabolismThauera aromaticaDemethylation |
| Journal | Chemistry in ecology (Print) |
| Editor | Gordon and Breach Science Publishers., New York, Stati Uniti d'America |
| Year | 2006 |
| Type | Articolo in rivista |
| DOI | 10.1080/02757540600557975 |
| Authors | A. Dibenedetto1; R.M. Lo Noce2; M. Narracci; M3. Aresta123 |
| Text | 6814 2006 10.1080/02757540600557975 ISI Web of Science WOS 000241198700010 Polyphenols; Anaerobic metabolism Thauera aromatica Demethylation Structure biodegradation correlation of polyphenols for Thauera aromatica K172 in anaerobic conditions A. Dibenedetto1; R.M. Lo Noce2; M. Narracci; M3. Aresta123 1Dip.to di Chimica, Universita di Bari, Campus Universitario, 70126 Bari, Italy 2Consorzio Interuniversitario Reattivita Chimica e Catalisi CIRCC Via Celso Ulpiani 27, 70126 Bari, Italy 3Istituto per l Ambiente Marino Costiero IAMC, Via Roma 3, 74100 Taranto, Italy The anaerobic degradation of phenolic compounds depends greatly on their molecular structure. We have undertaken a systematic study on several phenol derivatives which are commonly found in industrial waters with the aim of investigating the structure biodegradation relationship for bacteria in anaerobic conditions. The Thauera aromatica strain was used; this bacterium is able to use phenol as a sole carbon source in liquid cultures in the absence of molecular oxygen. Nitrate is the electron sink with complete reduction to N2. A systematic study has demonstrated the ability of this bacterial strain to use 3,4 dihydroxybenzoic acid, 4 hydroxycinnamic acid p coumaric acid , 4 hydroxy 3 methoxycinnamic acid ferulic acid , 3,4 dimethoxybenzoic acid veratric acid , and 3,4,5 trihydroxybenzoic acid gallic acid as substrate. The matching of the depletion of the concentration of such compounds with the increment in bacterial growth in the cultures demonstrates that the substrates are used as the sole carbon source. Also, a correlation between the rate of growth of bacteria and nitrite concentration has been observed. The progressive reduction in nitrate into nitrite and N2 is monitored through analyses. The protein pattern extracted from bacteria grown on methylated substrates is now being compared with that of bacteria grown on non methylated polyphenols that are structurally analogous in order to identify specific enzymes for demethylation processes. 22 Articolo pubblicato 2006 Structure_biodegradation_correlation_of_polyphenols_for_Thauera_aromatica_in_anaerobic_conditions.pdf Articolo in rivista Gordon and Breach Science Publishers. 0275 7540 Chemistry in ecology Print Chemistry in ecology Print Chem. ecol. Print Chemistry in ecology. Print Chemistry and ecology Print ARESTA MICHELE marcella.narracci NARRACCI MARCELLA TA.P04.002.001 Fascia Costiera e Ambienti di Transizione analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attivita |