| Title | Fluorescence In Situ Hybridization in soil and water ecosystems: an useful method for studying the effect of xenobiotics on bacterial community structure |
| Abstract | The ability of soil and groundwater ecosystems to recover from chemical
contamination is primarily dependent on the presence of a microbial
community which has the ability to remove it. Nevertheless, there has been
a little research into these communities because it is strictly dependent
on methods capable of identifying and characterizing their community
structure and functioning. The use of molecular methods makes it possible
to overcome this kind of identification limitation. In this work, we applied
the fluorescence in situ hybridization (FISH) method to different samples,
such as soil and groundwater contaminated with s-triazine herbicides
(simazine or terbuthylazine) and surface water treated with the pharmaceutical
oseltamivir carboxylate (Tamiflu). We compared the bacterial
community structure in the presence/absence of these xenobiotics. The use
of 16S rRNA-targeted oligonucleotide probes, designed specifically for
the main phylogenetic levels (Archaea, Bacteria, -, -, -, "-subdivision
of Proteobacteria, Planctomycetes, Gram-positive bacteria with a high or
low DNA GþC content, Cytophaga-Flavobacter-Bacteroides phylum,
and sulfate-reducing bacteria), and a DAPI stain made it possible to assess
the structure of the bacterial community and its changes in the presence
of these xenobiotics in all the ecosystems studied. |
| Source | Toxicological and environmental chemistry (Print) 92 (3), pp. 567–579 |
| Keywords | Bacterial phylogenetic characterizationGroundwaterOseltamivir carboxylate (tamiflu)Simazine and TerbuthylazineSoil and Surface water |
| Journal | Toxicological and environmental chemistry (Print) |
| Editor | Gordon and Breach Science Publishers., New York, Stati Uniti d'America |
| Year | 2010 |
| Type | Articolo in rivista |
| DOI | 10.1080/02772241003620244 |
| Authors | Barra Caracciolo A, Bottoni P, Grenni P |
| Text | 67872 2010 10.1080/02772241003620244 Scopus 2 s2.0 77949411140 Bacterial phylogenetic characterization Groundwater Oseltamivir carboxylate tamiflu Simazine and Terbuthylazine Soil and Surface water Fluorescence In Situ Hybridization in soil and water ecosystems an useful method for studying the effect of xenobiotics on bacterial community structure Barra Caracciolo A, Bottoni P, Grenni P BOTTONI P. Istituto Superiore di Sanita`, Viale Regina Elena 299 00166 Rome, Italy Barra Caracciolo A, Grenni P IRSA CNR The ability of soil and groundwater ecosystems to recover from chemical contamination is primarily dependent on the presence of a microbial community which has the ability to remove it. Nevertheless, there has been a little research into these communities because it is strictly dependent on methods capable of identifying and characterizing their community structure and functioning. The use of molecular methods makes it possible to overcome this kind of identification limitation. In this work, we applied the fluorescence in situ hybridization FISH method to different samples, such as soil and groundwater contaminated with s triazine herbicides simazine or terbuthylazine and surface water treated with the pharmaceutical oseltamivir carboxylate Tamiflu . We compared the bacterial community structure in the presence/absence of these xenobiotics. The use of 16S rRNA targeted oligonucleotide probes, designed specifically for the main phylogenetic levels Archaea, Bacteria, , , , subdivision of Proteobacteria, Planctomycetes, Gram positive bacteria with a high or low DNA GþC content, Cytophaga Flavobacter Bacteroides phylum, and sulfate reducing bacteria , and a DAPI stain made it possible to assess the structure of the bacterial community and its changes in the presence of these xenobiotics in all the ecosystems studied. 92 http //dx.doi.org/10.1080/02772241003620244 Articolo pubblicato TECFISH2010_pubblicazione.pdf Articolo in rivista Gordon and Breach Science Publishers. 0277 2248 Toxicological and environmental chemistry Print Toxicological and environmental chemistry Print Toxicol. environ. chem. Print Toxicological and environmental chemistry. Print anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.007 Strumenti di mitigazione dello stress quali quantitativo per i sistemi idrici |
