| Title | Natural microbial diversity in superficial sediments of Milazzo Harbor (Sicily) and community successions during microcosm enrichment with various hydrocarbons |
| Abstract | Hydrocarbon-contaminated superficial sediments collected from the Harbor of Milazzo (Tirrenean Sea, northern Sicily), a zone strongly affected by anthropogenic activities, were examined for in situ biodegradative capacities. A culture-independent molecular phylogenetic approach was used to study the influence of hydrocarbon and nutrient addition on the activity and diversity of the indigenous microbiota during a microcosm evaluation. The autochthonous microbial community in non-polluted sediments was represented by eubacterial phylotypes grouped within Proteobacteria, CFB and Firmicutes. The archaeal domain was represented by members of Marine Group I of Crenarchaeota. The majority of recovered sequences was affiliated with heterotrophic genera Clostridium and Vibrio, typical members of eutrophic coastal environments. Amendments of hydrocarbons and mineral nutrients to microcosms dramatically changed the initial diversity of the microbial community. Only bacterial phylotypes affiliated with Proteobacteria and CFB division were detected. The decrease in diversity observed in several microcosms could be explained by the strong selection for microorganisms belonging to group of marine hydrocarbonoclastic ³-Proteobacteria, namely Alcanivorax, Cycloclasticus, Marinobacter, Marinobacterium/Neptunomonas and Thalassolituus. This study demonstrated that nutrient amendment to hydrocarbon-contaminated superficial sediments enhanced the indigenous microbial biodegradation activity and that highly specialized marine hydrocarbonoclastic bacteria, representing a minor fraction in the natural microbial community, play an important role in the biodegradation of petroleum hydrocarbons accidentally entering the coastal environment. |
| Source | Environmental microbiology (Print) 7 (9), pp. 1426–1441 |
| Keywords | OBLIGATELY UTILIZES HYDROCARBONS; EXTRACTABLE ORGANIC-MATTER; OIL-SPILL BIOREMEDIATION; MARINE BACTERIUM; GEN. NOV.; CRUDE-OIL; SEA-WATER; BACTERIOPLANKTON COMMUNITIES; PETROLEUM-HYDROCARBONS; ALCANIVORAX STRAINS |
| Journal | Environmental microbiology (Print) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2005 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1462-2920.2005.00829.x |
| Authors | M.M. YAKIMOV, DENARO, R., GENOVESE, M., CAPPELLO, S., D'AURIA, G., CHERNIKOVA, T.N., TIMMIS, K.N., GOLYSHIN, P.N. AND GIULIANO, L. |
| Text | 6334 2005 10.1111/j.1462 2920.2005.00829.x ISI Web of Science WOS 000231024000015 PubMed 16104865 OBLIGATELY UTILIZES HYDROCARBONS; EXTRACTABLE ORGANIC MATTER; OIL SPILL BIOREMEDIATION; MARINE BACTERIUM; GEN. NOV.; CRUDE OIL; SEA WATER; BACTERIOPLANKTON COMMUNITIES; PETROLEUM HYDROCARBONS; ALCANIVORAX STRAINS Natural microbial diversity in superficial sediments of Milazzo Harbor Sicily and community successions during microcosm enrichment with various hydrocarbons M.M. YAKIMOV, DENARO, R., GENOVESE, M., CAPPELLO, S., D AURIA, G., CHERNIKOVA, T.N., TIMMIS, K.N., GOLYSHIN, P.N. AND GIULIANO, L. Tech Univ Carolo Wilhelmina Braunschweig, GBF, German Res Ctr Biotechnol, Div Microbiol, D 38106 Braunschweig, Germany Tech Univ Carolo Wilhelmina Braunschweig, Biozentrum, Inst Microbiol, D 38106 Braunschweig, Germany CNR, IAMC, Sez Messina, Ist Ambiente Marino Costiere, Messina, Italy Hydrocarbon contaminated superficial sediments collected from the Harbor of Milazzo Tirrenean Sea, northern Sicily , a zone strongly affected by anthropogenic activities, were examined for in situ biodegradative capacities. A culture independent molecular phylogenetic approach was used to study the influence of hydrocarbon and nutrient addition on the activity and diversity of the indigenous microbiota during a microcosm evaluation. The autochthonous microbial community in non polluted sediments was represented by eubacterial phylotypes grouped within Proteobacteria, CFB and Firmicutes. The archaeal domain was represented by members of Marine Group I of Crenarchaeota. The majority of recovered sequences was affiliated with heterotrophic genera Clostridium and Vibrio, typical members of eutrophic coastal environments. Amendments of hydrocarbons and mineral nutrients to microcosms dramatically changed the initial diversity of the microbial community. Only bacterial phylotypes affiliated with Proteobacteria and CFB division were detected. The decrease in diversity observed in several microcosms could be explained by the strong selection for microorganisms belonging to group of marine hydrocarbonoclastic ³ Proteobacteria, namely Alcanivorax, Cycloclasticus, Marinobacter, Marinobacterium/Neptunomonas and Thalassolituus. This study demonstrated that nutrient amendment to hydrocarbon contaminated superficial sediments enhanced the indigenous microbial biodegradation activity and that highly specialized marine hydrocarbonoclastic bacteria, representing a minor fraction in the natural microbial community, play an important role in the biodegradation of petroleum hydrocarbons accidentally entering the coastal environment. 7 http //onlinelibrary.wiley.com/doi/10.1111/j.1462 5822.2005.00829.x/pdf Natural microbial diversity in superficial sediments of Yakimov_et_al_EMI_2005.pdf Articolo in rivista Blackwell Science. 1462 2912 Environmental microbiology Print Environmental microbiology Print Environ. microbiol. Print simone.cappello CAPPELLO SIMONE maria.genovese GENOVESE MARIA laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA TA.P04.026.002 Impatto Antropogenico su ecosistemi marini microbiologia ed biotecnologia ambientale |
