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DatoValore
TitleNew methodological strategies for detecting bacterial indicators.
AbstractThe development of monitoring strategies for the early warning of seawater pollution, with particular reference to faecal and hydrocarbon contamination, has been the specific goal of research carried out within the Cluster 10-SAM (Advanced Systems for Coastal Marine Monitoring) Project, funded by the Italian Ministry for University and Scientific Research. Advanced analytical approaches have been designed and applied to detect bacterial species that have been selected as potential indicators of pollution in seawater samples. We report the results obtained using the fluorescent antibody and enzymatic assays for the detection of Escherichia coli, and a real-time PCR protocol for monitoring marine areas for hydrocarbon pollution. Immunofluorescence and enzymatic methods revealed the occurrence of different faecal pollution levels, reaching 105 E.coli cells 100 ml-1 in the Straits of Messina. Real-time PCR results corresponded to the different degree of oil pollution of the analysed samples. The specificity and speed make these methods promising for the detection and evaluation of marine pollution.
SourceChemistry and ecology (Online) 20, pp. 167–181
KeywordsMarine pollutionbacterial indicatorscoastal monitoring
JournalChemistry and ecology (Online)
EditorGordon and Breach Publishing Group., Amsterdam, Paesi Bassi
Year2004
TypeArticolo in rivista
DOI10.1080/02757540410001690333
AuthorsCaruso G., Denaro R., Genovese M., Giuliano L., Mancuso M., Yakimov M.M.
Text59866 2004 10.1080/02757540410001690333 Scopus 2 s2.0 5044238954 Marine pollution bacterial indicators coastal monitoring New methodological strategies for detecting bacterial indicators. Caruso G., Denaro R., Genovese M., Giuliano L., Mancuso M., Yakimov M.M. CNR IAMC MESSINA The development of monitoring strategies for the early warning of seawater pollution, with particular reference to faecal and hydrocarbon contamination, has been the specific goal of research carried out within the Cluster 10 SAM Advanced Systems for Coastal Marine Monitoring Project, funded by the Italian Ministry for University and Scientific Research. Advanced analytical approaches have been designed and applied to detect bacterial species that have been selected as potential indicators of pollution in seawater samples. We report the results obtained using the fluorescent antibody and enzymatic assays for the detection of Escherichia coli, and a real time PCR protocol for monitoring marine areas for hydrocarbon pollution. Immunofluorescence and enzymatic methods revealed the occurrence of different faecal pollution levels, reaching 105 E.coli cells 100 ml 1 in the Straits of Messina. Real time PCR results corresponded to the different degree of oil pollution of the analysed samples. The specificity and speed make these methods promising for the detection and evaluation of marine pollution. 20 prodotto con 6 citazioni su SCOPUS, 6 citazioni su Google Scholar, SJR 2011 0.284 SJR 2009 0.249, IF rivista 2009 0.634 New methodological strategies for detecting bacterial indicators GCHE041022.pdf Articolo in rivista Gordon and Breach Publishing Group. 1029 0370 Chemistry and ecology Online Chemistry and ecology Online Chem. ecol. Online gabriella.caruso CARUSO GABRIELLA laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA monique.mancuso MANCUSO MONIQUE TA.P03.003.001 Qualita del Mare e delle Acque Costiere