Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Ultraviolet radiation induce filamentation in bacterial assemblages from North Andean Patagonian lakes |
| Abstract | Through laboratory experiments, we tested whether UV radiation (UVR) induces filamentation in natural bacteria assemblages from North Andean Patagonian lakes. We incubated water from three different lakes for 72 h in four separate treatments: (1) UVR + PAR (photosynthetically active radiation), (2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. The irradiance levels used in the experiments were equivalent to those registered at the epilimnion of the lakes. In the UVR treatments filamentation was induced after the first 24 h and the proportion continued to increase for the next 48-72 h. A comparison of the gross composition and diversity of the entire community (cells >0.2 lm) with bacterial filaments alone (>5.0 lm) showed that UVR-induced filamentation is not a feature of any particular cluster. By sequencing part of the 16S rRNA gene of the taxonomic units obtained using denaturing gels, we observed that strains in the b-Proteobacteria group were of relatively high importance in filament formation, followed by Cytophaga-Flavobacterium-Bacteroides, c-Proteobacteria and a-Proteobacteria, whereas Actinobacteria were almost nonexistent in the filaments. We propose that UVR doses equivalent to those of Andean lakes produce bacterial morphological changes, and that all bacterial groups except Actinobacteria can potentially form filaments. |
| Source | Photochemistry and photobiology 86, pp. 871–881 |
| Journal | Photochemistry and photobiology |
| Editor | Blackwell., Oxford, Stati Uniti d'America |
| Year | 2010 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1751-1097.2010.00758.x |
| Authors | Modenutti B. (1), E. Balseiro (1), G. Corno, C. Callieri, R. Bertoni and E. Caravati |
| Text | 53183 2010 10.1111/j.1751 1097.2010.00758.x ISI Web of Science WOS 000279670500021 Ultraviolet radiation induce filamentation in bacterial assemblages from North Andean Patagonian lakes Modenutti B. 1 , E. Balseiro 1 , G. Corno, C. Callieri, R. Bertoni and E. Caravati 1 Laboratorio de Limnologia, INIBIOMA CONICET UNComahue , Quintral 1250, Bariloche, Argentina Through laboratory experiments, we tested whether UV radiation UVR induces filamentation in natural bacteria assemblages from North Andean Patagonian lakes. We incubated water from three different lakes for 72 h in four separate treatments 1 UVR PAR photosynthetically active radiation , 2 50% UVR PAR, 3 PAR and 4 50% PAR. The irradiance levels used in the experiments were equivalent to those registered at the epilimnion of the lakes. In the UVR treatments filamentation was induced after the first 24 h and the proportion continued to increase for the next 48 72 h. A comparison of the gross composition and diversity of the entire community cells >0.2 lm with bacterial filaments alone >5.0 lm showed that UVR induced filamentation is not a feature of any particular cluster. By sequencing part of the 16S rRNA gene of the taxonomic units obtained using denaturing gels, we observed that strains in the b Proteobacteria group were of relatively high importance in filament formation, followed by Cytophaga Flavobacterium Bacteroides, c Proteobacteria and a Proteobacteria, whereas Actinobacteria were almost nonexistent in the filaments. We propose that UVR doses equivalent to those of Andean lakes produce bacterial morphological changes, and that all bacterial groups except Actinobacteria can potentially form filaments. 86 Articolo pubblicato 3_Modenutti_et_al_2010_PCP.pdf Articolo in rivista Blackwell. 0031 8655 Photochemistry and photobiology Photochemistry and photobiology Photochem. photobiol. Photochemistry and photobiology. CARAVATI EMANUELE roberto.bertoni BERTONI ROBERTO cristiana.callieri CALLIERI CRISTIANA gianluca.corno CORNO GIANLUCA TA.P02.014.002 Impatto dei cambiamenti globali sugli ecosistemi acquatici |