| Title | Invasion genetics of the Eurasian spiny waterflea: Evidence for bottlenecks and gene flow using microsatellite data |
| Abstract | The Eurasian spiny waterflea (
Bythotrephes longimanus
) is a predacious zooplankter that
has increased its range in Europe and is rapidly invading inland water-bodies throughout
North America's Great Lakes region. To examine the genetics of these invasions, we isolated
five microsatellite DNA loci with between 5 and 19 alleles per locus. We sampled
three populations where
B. longimanus
has been historically present (Switzerland, Italy,
and Finland) as well as an introduced European population (the Netherlands) and three
North American populations (Lakes Erie, Superior, Shebandowan). Consistent with a bottleneck
during colonization (i.e. founder effect), average heterozygosities of the four European
populations ranged from 0.310 to 0.599, and were higher than that of three North
American populations (0.151-0.220). Pairwise
F
ST
estimates among North American populations
(0.002-0.063) were not significantly different from zero and were much lower than
among European populations (0.208-0.474). This is consistent with a scenario of high gene
flow among North American populations relative to that of European ones. Contrary to an
invasion bottleneck, however, Erie and Superior populations contained similar numbers of
rare alleles as European populations. Assignment tests identified several migrant genotypes
in all introduced populations (the Netherlands, Erie, Superior, Shebandowan), but
rarely in native ones (Switzerland, Italy and Finland). A large number of genotypes from
North America were assigned to our Italian population suggesting a second, previously
unidentified, invasion source somewhere in the region of northern Italy. Together, our
results support an invasion bottleneck for North American populations that has been
largely offset by gene flow from multiple native sources, as well as gene flow among introduced
populations. |
| Source | Molecular ecology (Print) 14, pp. 1869–1879 |
| Keywords | Bythotrephes longimanusgenetic diversityinvasion geneticsinvasivemicrosatellite |
| Journal | Molecular ecology (Print) |
| Editor | Blackwell Scientific Publications,, Oxford, Regno Unito |
| Year | 2005 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1365-294X.2005.02565.x |
| Authors | Colautti R.I., Manca M., Viljanen M., Ketelaars H.A.M., Bürgi H., MacIsaac H.J., Heath D.D. |
| Text | 52863 2005 10.1111/j.1365 294X.2005.02565.x ISI Web of Science WOS 000229190300003 Bythotrephes longimanus genetic diversity invasion genetics invasive microsatellite Invasion genetics of the Eurasian spiny waterflea Evidence for bottlenecks and gene flow using microsatellite data Colautti R.I., Manca M., Viljanen M., Ketelaars H.A.M., Burgi H., MacIsaac H.J., Heath D.D. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Ave., Windsor, ON N9B 3P4 Canada, CNR Istituto per lo Studio degli Ecosistemi, Largo Tonolli 50, 28922 Verbania, Italy, Karelian Institute, Department of Ecology, University of Joensuu, PO Box 111, FIN 80101 Joensuu, Finland, § Evides Water Company, PO BOX 59999, NL 3008 RA Rotterdam, The Netherlands, ¶ Swiss Federal Institute for Water Resources and Water Pollution Control, Ch 8600 Dubendorf, Switzerland The Eurasian spiny waterflea Bythotrephes longimanus is a predacious zooplankter that has increased its range in Europe and is rapidly invading inland water bodies throughout North America s Great Lakes region. To examine the genetics of these invasions, we isolated five microsatellite DNA loci with between 5 and 19 alleles per locus. We sampled three populations where B. longimanus has been historically present Switzerland, Italy, and Finland as well as an introduced European population the Netherlands and three North American populations Lakes Erie, Superior, Shebandowan . Consistent with a bottleneck during colonization i.e. founder effect , average heterozygosities of the four European populations ranged from 0.310 to 0.599, and were higher than that of three North American populations 0.151 0.220 . Pairwise F ST estimates among North American populations 0.002 0.063 were not significantly different from zero and were much lower than among European populations 0.208 0.474 . This is consistent with a scenario of high gene flow among North American populations relative to that of European ones. Contrary to an invasion bottleneck, however, Erie and Superior populations contained similar numbers of rare alleles as European populations. Assignment tests identified several migrant genotypes in all introduced populations the Netherlands, Erie, Superior, Shebandowan , but rarely in native ones Switzerland, Italy and Finland . A large number of genotypes from North America were assigned to our Italian population suggesting a second, previously unidentified, invasion source somewhere in the region of northern Italy. Together, our results support an invasion bottleneck for North American populations that has been largely offset by gene flow from multiple native sources, as well as gene flow among introduced populations. 14 Invasion genetics of the Eurasian spiny waterflea evidence Colautti2005.pdf Articolo in rivista Blackwell Scientific Publications, 0962 1083 Molecular ecology Print Molecular ecology Print Mol. ecol. Print Molecular ecology. Print marinamarcella.manca MANCA MARINA MARCELLA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |
