| Title | Bacteriophages limitedly contribute to the antimicrobial resistome of microbial communities in wastewater treatment plants |
| Abstract | Bacteriophages are known as players in the transmission of antimicrobial resistance genes (ARGs) by horizontal gene transfer. In this study, we characterized the bacteriophage community and the associated ARGs to estimate the potential for phages to spread ARGs in aquatic ecosystems analyzing the intra- and extracellular DNA isolated from two wastewater treatment plants (WWTPs) by shotgun metagenomics. We compared the phage antimicrobial resistome with the bacterial resistome and investigated the effect of the final disinfection treatment on the phage community and its resistome. Phage community was mainly composed by Siphoviridae and other members of the order Caudovirales. The final disinfection only marginally affected the composition of the phage community, and it was not possible to measure its effect on the antimicrobial resistome. Indeed, only three phage metagenome-assembled genomes (pMAGs) annotated as Siphoviridae, Padoviridae, and Myoviridae were positive for putative ARGs. Among the detected ARGs, i.e., dfrB6, rpoB mutants, and EF-Tu mutants, the first one was not annotated in the bacterial MAGs. Overall, these results demonstrate that bacteriophages limitedly contribute to the whole antimicrobial resistome. However, in order to obtain a comprehensive understanding of the antimicrobial resistome within a microbial community, the role of bacteriophages needs to be investigated. |
| Source | Microbiology spectrum |
| Keywords | antimicrobial resistomewastewater treatment plantsBacteriophages |
| Journal | Microbiology spectrum |
| Editor | ASM Press, Washington, DC, Stati Uniti d'America |
| Year | 2023 |
| Type | Articolo in rivista |
| DOI | 10.1128/spectrum.01101-23 |
| Authors | Raffaella Sabatino, Tomasa Sbaffi, Periyasamy Sivalingam, Gianluca Corno, Diego Fontaneto, Andrea Di Cesare |
| Text | 487159 2023 10.1128/spectrum.01101 23 antimicrobial resistome wastewater treatment plants Bacteriophages Bacteriophages limitedly contribute to the antimicrobial resistome of microbial communities in wastewater treatment plants Raffaella Sabatino, Tomasa Sbaffi, Periyasamy Sivalingam, Gianluca Corno, Diego Fontaneto, Andrea Di Cesare Molecular Ecology Group MEG , National Research Council of Italy Water Research Institute CNR IRSA , Verbania, Italy Bacteriophages are known as players in the transmission of antimicrobial resistance genes ARGs by horizontal gene transfer. In this study, we characterized the bacteriophage community and the associated ARGs to estimate the potential for phages to spread ARGs in aquatic ecosystems analyzing the intra and extracellular DNA isolated from two wastewater treatment plants WWTPs by shotgun metagenomics. We compared the phage antimicrobial resistome with the bacterial resistome and investigated the effect of the final disinfection treatment on the phage community and its resistome. Phage community was mainly composed by Siphoviridae and other members of the order Caudovirales. The final disinfection only marginally affected the composition of the phage community, and it was not possible to measure its effect on the antimicrobial resistome. Indeed, only three phage metagenome assembled genomes pMAGs annotated as Siphoviridae, Padoviridae, and Myoviridae were positive for putative ARGs. Among the detected ARGs, i.e., dfrB6, rpoB mutants, and EF Tu mutants, the first one was not annotated in the bacterial MAGs. Overall, these results demonstrate that bacteriophages limitedly contribute to the whole antimicrobial resistome. However, in order to obtain a comprehensive understanding of the antimicrobial resistome within a microbial community, the role of bacteriophages needs to be investigated. Published version https //journals.asm.org/doi/10.1128/spectrum.01101 23 phages 2023_MIcrSpectr_Sabatino et al EV.pdf Articolo in rivista ASM Press 2165 0497 Microbiology spectrum Microbiology spectrum Microbiology spectrum Microbiol. spectr. PERIYASAMY SIVALINGAM gianluca.corno CORNO GIANLUCA diego.fontaneto FONTANETO DIEGO andrea.dicesare DI CESARE ANDREA SABATINO RAFFAELLA SBAFFI TOMASA REDTEAR The Role of free Extracellular DNA in determining The Environmental Antibiotic Resistome DTA.AD002.661.001 REDTEAR SAC.AD002.173.039 Progetti@CNR ECOTREAT IRSA |
