| Title | Pilot-scale concentration and partial purification of food-grade phycocyanin from Arthrospira platensis via cross flow filtration: From biomass to final product |
| Abstract | From food- to analytical-grade products, the commercial exploitation of phycocyanin (PC) strongly depends on its degree of purity. In this study, we utilized a pilot-scale system (100 L working volume), based on serial tangential flow micro- and ultra-filtration (cut size 0.2 mu m and 50 kDa, respectively) for the concentration and purification of PC extracted from a commercial strain of Arthrospira platensis. The process resulted in a PC recovery of around 54%, whilst the resulting solution had a concentration of 1.1 +/- 0.2 mg(PC) mL(-1), purity of 1 +/- 0.2, and antioxidant activity of 84 +/- 6 ppm(ascorbic-acid-equivalent). Various preservatives were evaluated in order to enhance the stability of aqueous PC solution at room temperature. The addition of 0.4% citric acid had no effect (half-life similar to 7 days), while the addition of 2.5% sodium chloride or 20% sucrose significantly increased half-life up to similar to 250 days. PC solutions underwent either spray- or freeze-drying, either with or without carrier (trehalose) addition. The obtained PC powders had up to twofold higher color values compared to a commercial sample. The spray-drying process negatively affected the purity and yield of the final product (10% and 50% less than freeze-drying, respectively). In summary, our results suggest that tangential flow filtration followed by freeze-drying has huge potential for the production of food-grade phycocyanin. |
| Source | Journal of applied phycology |
| Keywords | Arthrospira platensisPhycocyaninCrossflow filtrationTangential filtrationStabilityDrying |
| Journal | Journal of applied phycology |
| Editor | Kluwer Academic Publishers, Dordrecht, Paesi Bassi |
| Year | 2023 |
| Type | Articolo in rivista |
| DOI | 10.1007/s10811-023-03014-y |
| Authors | Kurpan, Daniel; Ida, Antonio; Korner, Federico; Lauceri, Rosaria; Rocculi, Pietro; Phillips, Richard; Schievano, Andrea |
| Text | 486065 2023 10.1007/s10811 023 03014 y ISI Web of Science WOS 001006874400001 Arthrospira platensis Phycocyanin Crossflow filtration Tangential filtration Stability Drying Pilot scale concentration and partial purification of food grade phycocyanin from Arthrospira platensis via cross flow filtration From biomass to final product Kurpan, Daniel; Ida, Antonio; Korner, Federico; Lauceri, Rosaria; Rocculi, Pietro; Phillips, Richard; Schievano, Andrea Univ Milan; Algaria Srl; CNR; Univ Bologna; Membranology Ltd From food to analytical grade products, the commercial exploitation of phycocyanin PC strongly depends on its degree of purity. In this study, we utilized a pilot scale system 100 L working volume , based on serial tangential flow micro and ultra filtration cut size 0.2 mu m and 50 kDa, respectively for the concentration and purification of PC extracted from a commercial strain of Arthrospira platensis. The process resulted in a PC recovery of around 54%, whilst the resulting solution had a concentration of 1.1 / 0.2 mg PC mL 1 , purity of 1 / 0.2, and antioxidant activity of 84 / 6 ppm ascorbic acid equivalent . Various preservatives were evaluated in order to enhance the stability of aqueous PC solution at room temperature. The addition of 0.4% citric acid had no effect half life similar to 7 days , while the addition of 2.5% sodium chloride or 20% sucrose significantly increased half life up to similar to 250 days. PC solutions underwent either spray or freeze drying, either with or without carrier trehalose addition. The obtained PC powders had up to twofold higher color values compared to a commercial sample. The spray drying process negatively affected the purity and yield of the final product 10% and 50% less than freeze drying, respectively . In summary, our results suggest that tangential flow filtration followed by freeze drying has huge potential for the production of food grade phycocyanin. Published version https //link.springer.com/article/10.1007/s10811 023 03014 y 03/06/2023 Pilot scale concentration and partial purification of food grade phycocyanin from Arthrospira platensis via cross flow filtration Articolo 1 2023 KURPANetal Pilot scale concentration and purification of food grade PC.pdf Articolo in rivista Kluwer Academic Publishers 0921 8971 Journal of applied phycology Journal of applied phycology J. appl. phycol. Journal of applied phycology. rosaria.lauceri LAUCERI ROSARIA |
