| Title | Ecology of food waste chain-elongating microbiome |
| Abstract | Microbial chain elongation has emerged as a valuable bioprocess for obtaining marketable products, such as medium chain fatty acids usable in several industrial applications, from organic waste. The understanding of the microbiology and microbial ecology in these systems is crucial to apply these microbiomes in reliable production processes controlling microbial pathways to promote favourable metabolic processes, which will in turn increase product specificity and yields. In this research, the dynamics, cooperation/competition and potentialities of bacterial communities involved in the long-term lactate-based chain elongation process from food waste extract were evaluated under different operating conditions by DNA/RNA amplicon sequencing and functional profile prediction. The feeding strategies and the applied organic loading rates strongly affected the microbial community composition. The use of food waste extract promoted the selection of primary fermenters (i.e., Olsenella, Lactobacillus) responsible for the in situ production of electron donors (i.e., lactate). The discontinuous feeding and the organic loading rate 15 gCOD L d selected the best performing microbiome in which microbes coexist and cooperate to complete the chain elongation process. Both at DNA and RNA level, this microbiome was composed by the lactate producer Olsenella, the short chain fatty acids producers Anaerostipes, Clostridium sensu stricto 7, C. sensu stricto 12, Corynebacterium, Erysipelotrichaceae UCG-004, F0332, Leuconostoc, and the chain elongator Caproiciproducens. This microbiome also showed the highest predicted abundance of short-chain acyl-CoA dehydrogenase, the functional enzyme responsible for the chain elongation process. The combined approach herein used allowed to study the microbial ecology of chain elongation process from food waste by identifying the main functional groups, establishing the presence of potential biotic interactions within the microbiomes, and predicting metabolic potentialities. This study provided pivotal indications for the selection of high-performance microbiome involved in caproate production from food waste that can serve as a basis for further improving system performance and engineering the process scale-up. |
| Source | Frontiers in Bioengineering and Biotechnology 11 |
| Keywords | microbiomemicrobial chain elongationbiotic interactionsmultivariate statisticsDNA/ RNA sequencingfunctional predictionmedium chain fatty acidscaproatefood waste |
| Journal | Frontiers in Bioengineering and Biotechnology |
| Editor | , , |
| Year | 2023 |
| Type | Articolo in rivista |
| DOI | 10.3389/fbioe.2023.1157243 |
| Authors | Crognale S.; Massimi A.; Sbicego M.; Braguglia C.M.; Gallipoli A.; Gazzola G.; Gianico A.; Tonanzi B.; Di Pippo F.; Rossetti S. |
| Text | 481090 2023 10.3389/fbioe.2023.1157243 Scopus 2 s2.0 85153504365 microbiome microbial chain elongation biotic interactions multivariate statistics DNA/ RNA sequencing functional prediction medium chain fatty acids caproate food waste Ecology of food waste chain elongating microbiome Crognale S.; Massimi A.; Sbicego M.; Braguglia C.M.; Gallipoli A.; Gazzola G.; Gianico A.; Tonanzi B.; Di Pippo F.; Rossetti S. Water Research Institute, National Research Council of Italy, CNR IRSA Istituto di ricerca sulle acque, Roma Microbial chain elongation has emerged as a valuable bioprocess for obtaining marketable products, such as medium chain fatty acids usable in several industrial applications, from organic waste. The understanding of the microbiology and microbial ecology in these systems is crucial to apply these microbiomes in reliable production processes controlling microbial pathways to promote favourable metabolic processes, which will in turn increase product specificity and yields. In this research, the dynamics, cooperation/competition and potentialities of bacterial communities involved in the long term lactate based chain elongation process from food waste extract were evaluated under different operating conditions by DNA/RNA amplicon sequencing and functional profile prediction. The feeding strategies and the applied organic loading rates strongly affected the microbial community composition. The use of food waste extract promoted the selection of primary fermenters i.e., Olsenella, Lactobacillus responsible for the in situ production of electron donors i.e., lactate . The discontinuous feeding and the organic loading rate 15 gCOD L d selected the best performing microbiome in which microbes coexist and cooperate to complete the chain elongation process. Both at DNA and RNA level, this microbiome was composed by the lactate producer Olsenella, the short chain fatty acids producers Anaerostipes, Clostridium sensu stricto 7, C. sensu stricto 12, Corynebacterium, Erysipelotrichaceae UCG 004, F0332, Leuconostoc, and the chain elongator Caproiciproducens. This microbiome also showed the highest predicted abundance of short chain acyl CoA dehydrogenase, the functional enzyme responsible for the chain elongation process. The combined approach herein used allowed to study the microbial ecology of chain elongation process from food waste by identifying the main functional groups, establishing the presence of potential biotic interactions within the microbiomes, and predicting metabolic potentialities. This study provided pivotal indications for the selection of high performance microbiome involved in caproate production from food waste that can serve as a basis for further improving system performance and engineering the process scale up. 11 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85153504365 origin=inward Crognale et al., 2023 Crognale_2023_Frontiers.pdf Articolo in rivista 2296 4185 Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology TONANZI BARBARA GAZZOLA GIULIO MASSIMI ALESSIO simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA francesca.dipippo DI PIPPO FRANCESCA andrea.gianico GIANICO ANDREA agata.gallipoli GALLIPOLI AGATA simona.crognale CROGNALE SIMONA |
