| Abstract | Within the urban scenario, the application of a biorefinery technology value chain can foster the conversion of different organic substrates into marketable and added-value products. In this work, a pilot-scale dark fermentation (DF) process has been carried out as a key step for volatile fatty acids (VFA) and hydrogen production from the liquid fraction of sewage sludge and food waste mixture. Six operating conditions have been monitored in terms of yield and process stability, by changing the hydraulic retention time (HRT) from 4 to 6 days and applying a short-term hyper-thermophilic hydrolysis (70 °C, 8 h) on the same feedstock mixture. A tubular centrifugation was utilized to remove part of the biosolids (driven to biogas production) before the DF step, which was applied on the soluble and/or colloidal organic matter only. The hydrolysis step favored the following acidification process, in which a fermentation yield up to 0.42 g COD/g VS was achieved at 5 days as HRT. Hydrogen production (up to 34.4% v/v and 0.046 m H/kg VS) was positively affected by the hydrolysis application and by the decrease of the HRT, highlighting the possibility to produce biohythane in the modeled two-phases anaerobic bioprocess. On the other hand, without the application of the hydrolysis, a selective production of butyric acid (up to 75% COD basin) was achieved, furnishing a different valorization route for the chosen urban organic feedstock. Changes in operating conditions and performances were also reflected by the adaptation of the microbial community, whose characterization highlighted the occurrence of several fermentative microorganisms (e.g., Clostridiaceae, Ruminococcaceae). |
| Text | 481089 2023 10.1016/j.jclepro.2023.136919 Scopus 2 s2.0 85151057291 Dark fermentation DF Hydrolysis Butyrate Sewage sludge Hydrogen Boosting butyrate and hydrogen production in acidogenic fermentation of food waste and sewage sludge mixture a pilot scale demonstration Gottardo M.; Dosta J.; Cavinato C.; Crognale S.; Tonanzi B.; Rossetti S.; Bolzonella D.; Pavan P.; Valentino F. Department of Environmental Sciences, Informatics and Statistics, Ca Foscari University of Venice, Via Torino 155, 30170 Mestre Venice, Department of Environmental Sciences, Informatics and Statistics, Ca Foscari University of Venice, Via Torino 155, 30170 Mestre Venice, Italy, , Italy; Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, C/Marti i Franques 1, Barcelona, Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, C/Marti i Franques 1, Barcelona, Spain., , Spain; Water Research Institute, National Research Council of Italy, CNR IRSA, Via Salaria km 29.300, Monterotondo, Rome, 00015, Water Research Institute, National Research Council of Italy, CNR IRSA, Via Salaria km 29.300, 00015 Monterotondo, Rome, Italy, , , Italy; Water Research Institute, National Research Council of Italy, CNR IRSA, Via Salaria km 29.300, Monterotondo, Rome, 00015, Water Research Institute, National Research Council of Italy, CNR IRSA, Via Salaria km 29.300, 00015 Monterotondo, Rome, Italy, , , Italy; Department of Biotechnology, University of Verona, Strada Le Grazie 15, Verona, 37134, Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy, , Italy Within the urban scenario, the application of a biorefinery technology value chain can foster the conversion of different organic substrates into marketable and added value products. In this work, a pilot scale dark fermentation DF process has been carried out as a key step for volatile fatty acids VFA and hydrogen production from the liquid fraction of sewage sludge and food waste mixture. Six operating conditions have been monitored in terms of yield and process stability, by changing the hydraulic retention time HRT from 4 to 6 days and applying a short term hyper thermophilic hydrolysis 70 °C, 8 h on the same feedstock mixture. A tubular centrifugation was utilized to remove part of the biosolids driven to biogas production before the DF step, which was applied on the soluble and/or colloidal organic matter only. The hydrolysis step favored the following acidification process, in which a fermentation yield up to 0.42 g COD/g VS was achieved at 5 days as HRT. Hydrogen production up to 34.4% v/v and 0.046 m H/kg VS was positively affected by the hydrolysis application and by the decrease of the HRT, highlighting the possibility to produce biohythane in the modeled two phases anaerobic bioprocess. On the other hand, without the application of the hydrolysis, a selective production of butyric acid up to 75% COD basin was achieved, furnishing a different valorization route for the chosen urban organic feedstock. Changes in operating conditions and performances were also reflected by the adaptation of the microbial community, whose characterization highlighted the occurrence of several fermentative microorganisms e.g., Clostridiaceae, Ruminococcaceae . 404 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85151057291 origin=inward Gottardo et al., 2023 Gottardo_2023_boosting butyrate production.pdf Articolo in rivista Butterworth Heinemann, 0959 6526 Journal of cleaner production Journal of cleaner production J. clean. prod. Journal of cleaner production. TONANZI BARBARA simona.rossetti ROSSETTI SIMONA simona.crognale CROGNALE SIMONA |
