Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Extracellular DNA includes an important fraction of high-risk antibiotic resistance genes in treated wastewaters |
| Abstract | Wastewater treatment plants are among the main hotspots for the release of antibiotic resistance genes (ARGs) into the environment. ARGs in treated wastewater can be found in the intracellular DNA (iDNA) and in the extracellular DNA (eDNA). In this study, we investigated the fate and the distribution (either in eDNA or in iDNA) of ARGs in the treated wastewaters pre and post-disinfection by shotgun metagenomics. The richness of the intracellular resistome was found to be higher than the extracellular one. However, the latter included different high risk ARGs. About 11% of the recovered metagenome assembled genomes (MAGs) from the extracted DNA was positive for at least one ARG and, among them, several were positive for more ARGs. The high-risk ARG bacA was the most frequently detected gene among the MAGs. The disinfection demonstrated to be an important driver of the composition of the antibiotic resistomes. Our results demonstrated that eDNA represents an important fraction of the overall ARGs, including a number of high-risk ARGs, which reach the environment with treated wastewater effluents. The studied disinfections only marginally affect the whole antibiotic resistome but cause important shifts from intracellular to extracellular DNA, potentially threating human health. |
| Source | Environmental pollution (1987) 323 (121325) |
| Keywords | Antimicrobial resistanceExtracellular DNAMetagenomicsMAGDisinfection |
| Journal | Environmental pollution (1987) |
| Editor | Elsevier Applied Science Publishers, Barking, Regno Unito |
| Year | 2023 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.envpol.2023.121325 |
| Authors | Periyasamy Sivalingam, Raffaella Sabatino, Tomasa Sbaffi, Diego Fontaneto, Gianluca Corno, Andrea Di Cesare |
| Text | 478441 2023 10.1016/j.envpol.2023.121325 Antimicrobial resistance Extracellular DNA Metagenomics MAG Disinfection Extracellular DNA includes an important fraction of high risk antibiotic resistance genes in treated wastewaters Periyasamy Sivalingam, Raffaella Sabatino, Tomasa Sbaffi, Diego Fontaneto, Gianluca Corno, Andrea Di Cesare National Research Council of Italy Water Research Institute CNR IRSA , Molecular Ecology Group MEG , Verbania, Italy Wastewater treatment plants are among the main hotspots for the release of antibiotic resistance genes ARGs into the environment. ARGs in treated wastewater can be found in the intracellular DNA iDNA and in the extracellular DNA eDNA . In this study, we investigated the fate and the distribution either in eDNA or in iDNA of ARGs in the treated wastewaters pre and post disinfection by shotgun metagenomics. The richness of the intracellular resistome was found to be higher than the extracellular one. However, the latter included different high risk ARGs. About 11% of the recovered metagenome assembled genomes MAGs from the extracted DNA was positive for at least one ARG and, among them, several were positive for more ARGs. The high risk ARG bacA was the most frequently detected gene among the MAGs. The disinfection demonstrated to be an important driver of the composition of the antibiotic resistomes. Our results demonstrated that eDNA represents an important fraction of the overall ARGs, including a number of high risk ARGs, which reach the environment with treated wastewater effluents. The studied disinfections only marginally affect the whole antibiotic resistome but cause important shifts from intracellular to extracellular DNA, potentially threating human health. 323 Published version https //www.sciencedirect.com/science/article/pii/S0269749123003275 via%3Dihub siva eDNA 2023_EnvPoll_Sivalingam et al.pdf Articolo in rivista Elsevier Applied Science Publishers 0269 7491 Environmental pollution 1987 Environmental pollution 1987 Environ. pollut. 1987 Environmental pollution 1987 SABATINO RAFFAELLA SBAFFI TOMASA PERIYASAMY SIVALINGAM gianluca.corno CORNO GIANLUCA diego.fontaneto FONTANETO DIEGO andrea.dicesare DI CESARE ANDREA REDTEAR The Role of free Extracellular DNA in determining The Environmental Antibiotic Resistome |