| Title | Building a local reference library for metabarcoding survey of lake macrobenthos: oligochaetes and chironomids from Lake Maggiore |
| Abstract | This study represents a first reference database of genetic diversity of macroinvertebrates for a barcoding marker for Lake Maggiore, focusing on the two dominant groups of the littoral benthic fauna (chironomids and oligochaetes), commonly used for biological monitoring of freshwater lakes. Sediment samples were sorted at the stereomicroscope and single animals were cut in two pieces, one piece to be used for morphological identification and one piece for DNA extraction. This study allowed us to collect and identify 427 organisms: 309 oligochaetes belonging to 27 identifiable taxa and 118 chironomid larvae belonging to 26 identifiable taxa. Four families of oligochaetes: Naididae, Lumbricidae, Lumbriculidae, and Enchytraeidae and five subfamilies of Chironomidae: Chironominae, Tanypodinae, Orthocladiinae, Diamesinae, and Prodiamesinae were found. The extraction and amplification of the DNA covered a total of 10 oligochaete taxa. For 7 of them (Ophidonais serpentina, Uncinais uncinata, Vejdovskyella intermedia, Psammoryctides barbatus, Limnodrilus hoffmeisteri, Tubifex tubifex, and Bothrioneurum vejdovskyanum), we found other sequences in GenBank to compare genetic similarities with available data. For the other taxa (Lumbriculidae, and Enchytraeidae, and Nais sp.) no best hits were found in GenBank. The extraction and amplification of the DNA covered a total of 21 chironomid taxa. For ten species (Cladotanytarsus mancus, Cladotanytarsus atridorsum, Polypedilum scalaenum, Polypedilum nubeculosum, Benthalia carbonaria, Phaenopsectra flavipes, Clinotanypus nervosus, Paracladopelma laminatum, Cryptochironomus rostratus and Parakiefferiella finnmarkica) sequences were available in GenBank to compare genetic similarities. For the other taxa (Cryptochironomus sp., Demicryptochironomus vulneratus, Chironomus sp., Stictochironomus sp., Orthocladius sp., Cricotopus sp., Eukiefferiella sp., Procladius sp., Diamesa sp., Potthastia sp., and Monodiamesa bathyphila) no best hits were found in GenBank. For chironomids, DNA taxonomy revealed the existence of several species complexes. Covering more populations and more genetic markers for those taxa within a rationale of integrative taxonomy could solve the taxonomic problems and provide a reliable description of diversity. |
| Source | Advances in Oceanography and Limnology (Online) 13 (2) |
| Keywords | metabarcoding |
| Journal | Advances in Oceanography and Limnology (Online) |
| Editor | Taylor & Francis, 2010, Regno Unito |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.4081/aiol.2022.11051 |
| Authors | Silvia Zaupa, Diego Fontaneto, Raffaella Sabatino, Angela Boggero |
| Text | 477869 2022 10.4081/aiol.2022.11051 metabarcoding Building a local reference library for metabarcoding survey of lake macrobenthos oligochaetes and chironomids from Lake Maggiore Silvia Zaupa, Diego Fontaneto, Raffaella Sabatino, Angela Boggero National Research Council Water Research Institute CNR IRSA , Verbania National Biodiversity Future Center NBFC , Palermo, Italy. This study represents a first reference database of genetic diversity of macroinvertebrates for a barcoding marker for Lake Maggiore, focusing on the two dominant groups of the littoral benthic fauna chironomids and oligochaetes , commonly used for biological monitoring of freshwater lakes. Sediment samples were sorted at the stereomicroscope and single animals were cut in two pieces, one piece to be used for morphological identification and one piece for DNA extraction. This study allowed us to collect and identify 427 organisms 309 oligochaetes belonging to 27 identifiable taxa and 118 chironomid larvae belonging to 26 identifiable taxa. Four families of oligochaetes Naididae, Lumbricidae, Lumbriculidae, and Enchytraeidae and five subfamilies of Chironomidae Chironominae, Tanypodinae, Orthocladiinae, Diamesinae, and Prodiamesinae were found. The extraction and amplification of the DNA covered a total of 10 oligochaete taxa. For 7 of them Ophidonais serpentina, Uncinais uncinata, Vejdovskyella intermedia, Psammoryctides barbatus, Limnodrilus hoffmeisteri, Tubifex tubifex, and Bothrioneurum vejdovskyanum , we found other sequences in GenBank to compare genetic similarities with available data. For the other taxa Lumbriculidae, and Enchytraeidae, and Nais sp. no best hits were found in GenBank. The extraction and amplification of the DNA covered a total of 21 chironomid taxa. For ten species Cladotanytarsus mancus, Cladotanytarsus atridorsum, Polypedilum scalaenum, Polypedilum nubeculosum, Benthalia carbonaria, Phaenopsectra flavipes, Clinotanypus nervosus, Paracladopelma laminatum, Cryptochironomus rostratus and Parakiefferiella finnmarkica sequences were available in GenBank to compare genetic similarities. For the other taxa Cryptochironomus sp., Demicryptochironomus vulneratus, Chironomus sp., Stictochironomus sp., Orthocladius sp., Cricotopus sp., Eukiefferiella sp., Procladius sp., Diamesa sp., Potthastia sp., and Monodiamesa bathyphila no best hits were found in GenBank. For chironomids, DNA taxonomy revealed the existence of several species complexes. Covering more populations and more genetic markers for those taxa within a rationale of integrative taxonomy could solve the taxonomic problems and provide a reliable description of diversity. 13 Published version https //www.pagepressjournals.org/index.php/aiol/article/view/11051 zaupa aiol_2022_13 2 _11051_opt.pdf Articolo in rivista Taylor Francis 1947 573X Advances in Oceanography and Limnology Online Advances in Oceanography and Limnology Online Advances in Oceanography and Limnology ZAUPA SILVIA SABATINO RAFFAELLA angela.boggero BOGGERO ANGELA diego.fontaneto FONTANETO DIEGO DTA.AD002.700.001 CIPAIS RIVE 2022 2024 SAC.AD002.173.047 Progetti@CNR SOS Acque IRSA PRR.AP005.006.001 NBFC_SPOKE3_ ASSESSING AND MONITORING TERRESTRIAL AND FRESHWATER BIODIVERSITY AND ITS EVOLUTION FROM TAXONOMY TO GENOMICS AND CITIZEN SCIENCE_IRSA |
