| Title | Class 1 integron and related antimicrobial resistance gene dynamics along a complex freshwater system affected by different anthropogenic pressures |
| Abstract | The risk for human health posed by polluted aquatic environments, and especially those carrying antibiotic resistance genes (ARGs) of clinical interest, is still debated. This is because of our limited knowledge of the dynamics of antimicrobial resistance in the environment, the selection mechanisms underlying the spread of ARGs, and the ecological factors potentially favoring their return to humans. The Class 1 integron is one of the most effective platforms for the dissemination of ARGs. In this study we investigated a freshwater system consisting of a lake-river-lake continuum, determining the abundance of class 1 integrons and their associated ARGs by a modulated metagenomic approach. Bacterial abundance and community composition were used to identify the potential carriers of class 1 integrons and their associated ARGs over a period of six months. Class 1 integrons and their ARG cargoes were significantly more abundant in riverine sampling sites receiving treated wastewater. Further, class 1 integrons carried ARGs ranked at the highest risk for human health (e.g., catB genes), in particular, genes encoding resistance to aminoglycosides. Genera of potential pathogens, such as Pseudomonas and Escherichia-Shigella, were correlated with class 1 integrons. The lake-river-lake system demonstrated a clear relationship between the integrase gene of class 1 integrons (intI1) and anthropogenic impact, but also a strong environmental filtering that favored the elimination of intI1 once the human derived stressors were reduced. Overall, the results of this study underline the role class 1 integrons as proxy of anthropogenic pollution and suggest this genetic platform as an important driver of aminoglycoside resistance genes, including high risk ARGs, of potential concern for human health. |
| Source | Environmental pollution (1987) 316 |
| Keywords | Antimicrobial resistanceClass 1 integronsLakeRiverPathogenic bacteria |
| Journal | Environmental pollution (1987) |
| Editor | Elsevier Applied Science Publishers, Barking, Regno Unito |
| Year | 2023 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.envpol.2022.120601 |
| Authors | Corno G.; Ghaly T.; Sabatino R.; Eckert E.M.; Galafassi S.; Gillings M.R.; Di Cesare A. |
| Text | 477477 2023 10.1016/j.envpol.2022.120601 Scopus 2 s2.0 85141525973 Antimicrobial resistance Class 1 integrons Lake River Pathogenic bacteria Class 1 integron and related antimicrobial resistance gene dynamics along a complex freshwater system affected by different anthropogenic pressures Corno G.; Ghaly T.; Sabatino R.; Eckert E.M.; Galafassi S.; Gillings M.R.; Di Cesare A. National Research Council of Italy Water Research Institute CNR IRSA Molecular Ecology Group MEG , Verbania, National Research Council of Italy Water Research Institute CNR IRSA Molecular Ecology Group MEG , Verbania Italy, , Italy; ARC Centre of Excellence in Synthetic Biology and Department of Biological Sciences, Macquarie University, Sydney, NSW, ARC Centre of Excellence in Synthetic Biology and Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia, , Australia The risk for human health posed by polluted aquatic environments, and especially those carrying antibiotic resistance genes ARGs of clinical interest, is still debated. This is because of our limited knowledge of the dynamics of antimicrobial resistance in the environment, the selection mechanisms underlying the spread of ARGs, and the ecological factors potentially favoring their return to humans. The Class 1 integron is one of the most effective platforms for the dissemination of ARGs. In this study we investigated a freshwater system consisting of a lake river lake continuum, determining the abundance of class 1 integrons and their associated ARGs by a modulated metagenomic approach. Bacterial abundance and community composition were used to identify the potential carriers of class 1 integrons and their associated ARGs over a period of six months. Class 1 integrons and their ARG cargoes were significantly more abundant in riverine sampling sites receiving treated wastewater. Further, class 1 integrons carried ARGs ranked at the highest risk for human health e.g., catB genes , in particular, genes encoding resistance to aminoglycosides. Genera of potential pathogens, such as Pseudomonas and Escherichia Shigella, were correlated with class 1 integrons. The lake river lake system demonstrated a clear relationship between the integrase gene of class 1 integrons intI1 and anthropogenic impact, but also a strong environmental filtering that favored the elimination of intI1 once the human derived stressors were reduced. Overall, the results of this study underline the role class 1 integrons as proxy of anthropogenic pollution and suggest this genetic platform as an important driver of aminoglycoside resistance genes, including high risk ARGs, of potential concern for human health. 316 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85141525973 origin=inward Articolo in rivista Elsevier Applied Science Publishers 0269 7491 Environmental pollution 1987 Environmental pollution 1987 Environ. pollut. 1987 Environmental pollution 1987 gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA estermaria.eckert ECKERT ESTER MARIA silvia.galafassi GALAFASSI SILVIA |
