| Title | Antibiotic resistance genes load in an antibiotic free organic broiler farm |
| Abstract | Antibiotic resistance is a serious concern for public health. Farm environments are relevant reservoirs of antibiotic resistant bacteria and antibiotic resistance genes (ARGs), thus strategies to limit the spread of ARGs from farms to the environment are needed. In this study a broiler farm, where antibiotics have never been used for any purpose, was selected to evaluate if this measure is effective in reducing the ARGs load in farm environment (FE) and in meat processing environment (MPE). Faecal samples from FE and MPE were processed for DNA extraction. Detection and quantification of the 16S rRNA gene and selected ARGs (bla(TEM), qnrS, sul2, and tetA) were carried out by PCR and digital droplet PCR (ddPCR), respectively. Generally, the relative abundance of the quantified ARGs in FE was similar or higher than that measured in intensive farms. Furthermore, apart for tetA, no differences in relative abundances of the other ARGs between FE and MPE were determined. These results suggest that the choice to not use antibiotics in broiler farming is not so effective to limit the ARGs spread in MPE and that further sources of ARGs should be considered including the preceding production phase with particular reference to the breeding stage. |
| Source | Poultry science (Print) 101 (3) |
| Keywords | antibiotic resistanceantibiotic freepoultry farmddPCR |
| Journal | Poultry science (Print) |
| Editor | Poultry Science Association, etc.], [Champaign, IL, etc.,, Stati Uniti d'America |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.psj.2021.101675 |
| Authors | Salerno, Barbara; Furlan, Maddalena; Sabatino, Raffaella; Di Cesare, Andrea; Leati, Marta; Volanti, Marcello; Barco, Lisa; Orsini, Massimiliano; Losasso, Carmen; Cibin, Veronica |
| Text | 477473 2022 10.1016/j.psj.2021.101675 ISI Web of Science WOS 000791426400008 antibiotic resistance antibiotic free poultry farm ddPCR Antibiotic resistance genes load in an antibiotic free organic broiler farm Salerno, Barbara; Furlan, Maddalena; Sabatino, Raffaella; Di Cesare, Andrea; Leati, Marta; Volanti, Marcello; Barco, Lisa; Orsini, Massimiliano; Losasso, Carmen; Cibin, Veronica Ist Zooprofilatt Sperimentale Venezie; CNR Natl Res Council Italy Antibiotic resistance is a serious concern for public health. Farm environments are relevant reservoirs of antibiotic resistant bacteria and antibiotic resistance genes ARGs , thus strategies to limit the spread of ARGs from farms to the environment are needed. In this study a broiler farm, where antibiotics have never been used for any purpose, was selected to evaluate if this measure is effective in reducing the ARGs load in farm environment FE and in meat processing environment MPE . Faecal samples from FE and MPE were processed for DNA extraction. Detection and quantification of the 16S rRNA gene and selected ARGs bla TEM , qnrS, sul2, and tetA were carried out by PCR and digital droplet PCR ddPCR , respectively. Generally, the relative abundance of the quantified ARGs in FE was similar or higher than that measured in intensive farms. Furthermore, apart for tetA, no differences in relative abundances of the other ARGs between FE and MPE were determined. These results suggest that the choice to not use antibiotics in broiler farming is not so effective to limit the ARGs spread in MPE and that further sources of ARGs should be considered including the preceding production phase with particular reference to the breeding stage. 101 Published version Articolo in rivista Poultry Science Association, etc. 0032 5791 Poultry science Print Poultry science Print Poult. sci. Print Poultry science. Print SABATINO RAFFAELLA andrea.dicesare DI CESARE ANDREA |
