| Abstract | We investigated the performance of a heterogeneous Fenton-like reaction (ZVI-Fenton, i.e., ZVI + HO, where ZVI = zero-valent iron) towards the removal of potentially pathogenic bacteria in wastewater (WW). The effectiveness of the process was investigated towards live bacteria (measured by flow cytometry) as well as potentially pathogenic bacteria (identified by 16S rRNA amplicon sequencing), antibiotic resistance genes and class 1 integrons (assessed by qPCR). The ZVI-Fenton process resulted about as effective as HO alone to decrease live bacteria (p > 0.05), if compared with the blank controls (neither ZVI nor HO, p = 0.00005), although it did not reduce the relative abundance of the tested antibiotic resistance genes or class 1 integrons (p > 0.05). However, ZVI-Fenton was quite effective in lowering the total content of the potentially pathogenic bacteria in WW when compared to the controls with HO (p = 0.0186) and without HO (p = 0.0252). These findings suggest that ZVI-Fenton has potential as an effective WW treatment technique that should be further investigated for future application in WWTPs. |
| Text | 477472 2022 10.1016/j.jwpe.2022.102668 Scopus 2 s2.0 85125186594 Zero valent iron Fenton like process Wastewater Pathogens Antibiotic resistance genes Advanced oxidation processes The ZVI Fenton process affects the total load of human pathogenic bacteria in wastewater samples Sabatino R.; Furia F.; Eckert E.M.; Minella M.; Corno G.; Di Cesare A.; Vione D. Water Research Institute IRSA MEG Molecular Ecology Group, CNR National Research Council of Italy, Largo Tonolli 50, Verbania, 28922, Water Research Institute IRSA MEG Molecular Ecology Group, CNR National Research Council of Italy, Largo Tonolli 50, 28922, Verbania, Italy, , Italy; Dipartimento attivita produttive e impatto sul territorio UOC Valutazione e Pareri Ambientali Arpa Sicilia, Viale Cristoforo Colombo snc, Palermo, 90149, Dipartimento attivita produttive e impatto sul territorio UOC Valutazione e Pareri Ambientali Arpa Sicilia, Viale Cristoforo Colombo snc, 90149, Palermo, Italy, , Italy; Department of Chemistry, Universita di Torino, Via Pietro Giuria, 5, Torino, 10125, Department of Chemistry, Universita di Torino, Via Pietro Giuria, 5, 10125 Torino, Italy, , Italy We investigated the performance of a heterogeneous Fenton like reaction ZVI Fenton, i.e., ZVI HO, where ZVI = zero valent iron towards the removal of potentially pathogenic bacteria in wastewater WW . The effectiveness of the process was investigated towards live bacteria measured by flow cytometry as well as potentially pathogenic bacteria identified by 16S rRNA amplicon sequencing , antibiotic resistance genes and class 1 integrons assessed by qPCR . The ZVI Fenton process resulted about as effective as HO alone to decrease live bacteria p > 0.05 , if compared with the blank controls neither ZVI nor HO, p = 0.00005 , although it did not reduce the relative abundance of the tested antibiotic resistance genes or class 1 integrons p > 0.05 . However, ZVI Fenton was quite effective in lowering the total content of the potentially pathogenic bacteria in WW when compared to the controls with HO p = 0.0186 and without HO p = 0.0252 . These findings suggest that ZVI Fenton has potential as an effective WW treatment technique that should be further investigated for future application in WWTPs. 47 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85125186594 origin=inward Articolo in rivista Elsevier Journal of Water Process Engineering Journal of Water Process Engineering SABATINO RAFFAELLA gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA estermaria.eckert ECKERT ESTER MARIA |
