Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Occurrence of antibiotic-resistant bacteria and resistance genes in the urban water cycle |
| Abstract | This study investigates the antibiotic resistance fate in the urban water cycle, evaluating the dynamics of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in three different full-scale wastewater treatment plants (WWTPs) and two drinking water treatment plants (DWTPs) located in the same geographical area (North-West of Italy). ARB (tetracycline-, ampicillin-, and sulfonamide-resistant bacteria) were quantified by plate counting and the abundances of selected ARGs (i.e., tetA, bla(TEM), and sulII) and intI1 gene were measured using quantitative real-time PCR (qPCR). Higher concentrations of ARB and ARGs were observed in the WWTPs with respect to the DWTPs identifying the WWTP as hotspot for the spread of antibiotic resistances. Although a significant reduction of ARB and ARGs was observed in WWTPs and DWTPs after the treatment, none of the detected ARB or ARGs was completely removed in drinking water. The stability of the antibiotic-resistant rates between inlet and outlet associated with the reduction of relative ARG abundances underlined that both the treatments (WWTs and DWTs) did not apply any selective pressure. The overall results highlighted the importance to investigate the antibiotic resistance dynamics in aquatic ecosystems involved in urban water cycle integrating the information obtained by culture-dependent method with the culture-independent one and the need to monitor the presence of ARB and ARGs mainly in drinking water that represents a potential route of transmission to human. |
| Source | Environmental science and pollution research international |
| Keywords | Urban water cycleAntibiotic resistanceAntibiotic resistance bacteriaAntibiotic resistance genesWastewater treatmentDrinking water treatment |
| Journal | Environmental science and pollution research international |
| Editor | Springer, Berlin, Germania |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1007/s11356-022-24650-w |
| Authors | Bonetta, Silvia; Di Cesare, Andrea; Pignata, Cristina; Sabatino, Raffaella; Macri, Manuela; Corno, Gianluca; Panizzolo, Marco; Bonetta, Sara; Carraro, Elisabetta |
| Text | 477465 2022 10.1007/s11356 022 24650 w ISI Web of Science WOS 000900095900017 Urban water cycle Antibiotic resistance Antibiotic resistance bacteria Antibiotic resistance genes Wastewater treatment Drinking water treatment Occurrence of antibiotic resistant bacteria and resistance genes in the urban water cycle Bonetta, Silvia; Di Cesare, Andrea; Pignata, Cristina; Sabatino, Raffaella; Macri, Manuela; Corno, Gianluca; Panizzolo, Marco; Bonetta, Sara; Carraro, Elisabetta Univ Torino; Natl Res Council Italy; Univ Torino This study investigates the antibiotic resistance fate in the urban water cycle, evaluating the dynamics of antibiotic resistant bacteria ARB and antibiotic resistant genes ARGs in three different full scale wastewater treatment plants WWTPs and two drinking water treatment plants DWTPs located in the same geographical area North West of Italy . ARB tetracycline , ampicillin , and sulfonamide resistant bacteria were quantified by plate counting and the abundances of selected ARGs i.e., tetA, bla TEM , and sulII and intI1 gene were measured using quantitative real time PCR qPCR . Higher concentrations of ARB and ARGs were observed in the WWTPs with respect to the DWTPs identifying the WWTP as hotspot for the spread of antibiotic resistances. Although a significant reduction of ARB and ARGs was observed in WWTPs and DWTPs after the treatment, none of the detected ARB or ARGs was completely removed in drinking water. The stability of the antibiotic resistant rates between inlet and outlet associated with the reduction of relative ARG abundances underlined that both the treatments WWTs and DWTs did not apply any selective pressure. The overall results highlighted the importance to investigate the antibiotic resistance dynamics in aquatic ecosystems involved in urban water cycle integrating the information obtained by culture dependent method with the culture independent one and the need to monitor the presence of ARB and ARGs mainly in drinking water that represents a potential route of transmission to human. Published version Articolo in rivista Springer 0944 1344 Environmental science and pollution research international Environmental science and pollution research international Environ. sci. pollut. res. int. Environmental science and pollution research international. Environmental science and pollution research international Print Environmental science and pollution research Print ESPR Print SABATINO RAFFAELLA gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA |