| Text | 476817 2022 10.3897/mbmg.6.85652 Scopus 2 s2.0 85137246592 Aquatic ecosystems DNAqua Net Ecological assessment eDNA Water Framework Directive A strategy for successful integration of DNA based methods in aquatic monitoring Blancher P.; Lefrancois E.; Rimet F.; Vasselon V.; Argillier C.; Arle J.; Beja P.; Boets P.; Boughaba J.; Chauvin C.; Deacon M.; Duncan W.; Ejdung G.; Erba S.; Ferrari B.; Fischer H.; Hanfling B.; Haldin M.; Hering D.; Hette Tronquart N.; Hiley A.; Jarvinen M.; Jeannot B.; Kahlert M.; Kelly M.; Kleinteich J.; Koyuncuoglu S.; Krenek S.; Langhein Winther S.; Leese F.; Mann D.; Marcel R.; Marcheggiani S.; Meissner K.; Mergen P.; Monnier O.; Narendja F.; Neu D.; Pinto V.O.; Pawlowska A.; Pawlowski J.; Petersen M.; Poikane S.; Pont D.; Renevier M. S.; Sandoy S.; Svensson J.; Trobajo R.; Zagyva A.T.; Tziortzis I.; Van Der Hoorn B.; Vasquez M.I.; Walsh K.; Weigand A.; Bouchez A. Blancher Consultant, Lyon, Blancher Consultant, Lyon, France, , France; ECO in Eau, Montferrier sur Lez, ECO in EAU, Montferrier sur Lez, France, , France; INRAE, USMB, CARRTEL, Thonon les Bains, INRAE, USMB, CARRTEL, Thonon les Bains, France, , , France; INRAE, USMB, CARRTEL, Thonon les Bains, INRAE, USMB, CARRTEL, Thonon les Bains, France, , , France; Scimabio Interface, Thonon les Bains, Scimabio Interface, Thonon les Bains, France, , France; INRAE, RECOVER, Aix en Provence, INRAE, RECOVER, Aix en Provence, France, , , France; INRAE, RECOVER, Aix en Provence, INRAE, RECOVER, Aix en Provence, France, , , France; German Environment Agency, Dessau Roßlau, German Environment Agency, Dessau Roßlau, Germany, , Germany; CIBIO/InBio, University of Porto, Porto, CIBIO/InBio, University of Porto, Porto, Portugal, , , Portugal; CIBIO/InBio, University of Porto, Porto, CIBIO/InBio, University of Porto, Porto, Portugal, , , Portugal; Provincial Centre of Environmental Research, Ghent, Provincial Centre of Environmental Research, Ghent, Belgium, , Belgium; European Commission, Brussel, European Commission, Brussel, Belgium, , Belgium; INRAE, EABX, Cestas, INRAE, EABX, Cestas, France, , , France; INRAE, EABX, Cestas, INRAE, EABX, Cestas, France, , , France; Danish Environmental Protection Agency, Gram, Danish Environmental Protection Agency, Gram, Denmark, , Denmark; Scottish Environment Protection Agency, Stirling, Scottish Environment Protection Agency, Stirling, United Kingdom, , United Kingdom; Swedish Environmental Protection Agency SEPA , Lund, Swedish Environmental Protection Agency SEPA , Lund, Sweden, , Sweden; Water Research Institute, CNR IRSA, Brugherio, Water Research Institute, CNR IRSA, Brugherio, Italy, , Italy; Swiss Centre for Applied Ecotoxicology, Lausanne, Swiss Centre for Applied Ecotoxicology, Lausanne, Switzerland, , Switzerland; German Federal Institute of Hydrology, BfG, Koblenz, German Federal Institute of Hydrology, BfG, Koblenz, Germany, , Germany; University of Hull, Hull, University of Hull, Hull, United Kingdom, , United Kingdom; Swedish Agency for Marine and Water Management, Goteborg, Swedish Agency for Marine and Water Management, Goteborg, Sweden, , Sweden; University of Duisburg Essen, Duisburg Essen, Essen, University of Duisburg Essen, Duisburg Essen, Essen, Germany, , Germany; Office Français de la Biodiversite, OFB, Vincennes, Office Français de la Biodiversite, OFB, Vincennes, France, , France; UK Environment Agency, Rotherham, UK Environment Agency, Rotherham, United Kingdom, , United Kingdom; Finnish Environment Institute, SYKE, Jyvaskyla, Helsinki, Finnish Environment Institute, SYKE, Jyvaskyla, Helsinki, Finland, , Finland; French Ministry for An Ecological Transition, MET, Paris, French Ministry for an Ecological Transition, MET, Paris, France, , France; Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment, Uppsala, Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment, Uppsala, Sweden, , Sweden; Bowburn Consultancy, Bowburn, Bowburn Consultancy, Bowburn, United Kingdom, , United Kingdom; Water Management, MoAF, Clermont Ferrand, Ankara, Water Management, MoAF, Clermont Ferrand, Ankara, Turkey, , Turkey; Royal Botanic Garden Edinburgh, Edinburgh, Royal Botanic Garden Edinburgh, Edinburgh, United Kingdom, , United Kingdom; Aquabio, Clermont Ferrand, Aquabio, Clermont Ferrand, France, , France; Italian National Institute of Health, Roma, Italian National Institute of Health, Roma, Italy, , Italy; Meise Botanic Garden, Meise, Meise Botanic Garden, Meise, Belgium, , Belgium; Environment Agency, Vienna, Environment Agency, Vienna, Austria, , Austria; Administration de la Gestion de l Eau, AGE, Luxembourg, Administration de la gestion de l eau, AGE, Luxembourg, , Luxembourg; Portuguese Environment Agency, Amadora, Portuguese Environment Agency, Amadora, Portugal, , Portugal; ID Gene Ecodiagnostics, Plan les Ouates, ID Gene ecodiagnostics, Plan les Ouates, Switzerland, , Switzerland; University of Geneva, Geneva, University of Geneva, Geneva, Switzerland, , Switzerland; European Commission, Joint Research Centre JRC , Ispra, European Commission, Joint Research Centre JRC , Ispra, Italy, , Italy; BOKU IHG, Vienna, BOKU IHG, Vienna, Austria, , Austria; Federal Office for the Environment, FOEN, Federal Office for the Environment, FOEN, Switzerland, , Switzerland; Noregian Environment Agency, Trondheim, Norwegian Environment Agency, Trondheim, Norway, , Norway; Institute for Food and Agricultural Research and Technology, IRTA, Sant Carles de la Rapita, Catalonia, Institute for Food and Agricultural Research and Technology, IRTA, Sant Carles de la Rapita, Catalonia, Spain, , Spain; Department for Water Management and Water, OVF, Department for Water Management and Water, OVF, Hungary, , Hungary; Water Development Department, Ministry of Agriculture, Rural Development and Environment, Water Development Department, Ministry of Agriculture, Rural Development and Environment, Cyprus, , Cyprus; Inholland University of Applied Sciences, Delft, Leiden, Inholland University of Applied Sciences, Delft, Leiden, Netherlands, , Netherlands; Cyprus University of Technology, CUT, Limassol, Cyprus University of Technology, CUT, Limassol, Cyprus, , Cyprus; National Museum of Natural History Luxembourg, MNHNL, National Museum of Natural History Luxembourg, MNHNL, Luxembourg, , Luxembourg Recent advances in molecular biomonitoring open new horizons for aquatic ecosystem assessment. Rapid and cost effective methods based on organismal DNA or environmental DNA eDNA now offer the opportunity to produce inventories of indicator taxa that can subsequently be used to assess biodiversity and ecological quality. However, the integration of these new DNA based methods into current monitoring practices is not straightforward, and will require coordinated actions in the coming years at national and international levels. To plan and stimulate such an integration, the European network DNAqua Net COST Action CA15219 brought together international experts from academia, as well as key environmental biomonitoring stakeholders from different European countries. Together, this transdisciplinary consortium developed a roadmap for implementing DNA based methods with a focus on inland waters assessed by the EU Water Framework Directive 2000/60/EC . This was done through a series of online workshops held in April 2020, which included fifty participants, followed by extensive synthesis work. The roadmap is organised around six objectives 1 to highlight the effectiveness and benefits of DNA based methods, 2 develop an adaptive approach for the implementation of new methods, 3 provide guidelines and standards for best practice, 4 engage stakeholders and ensure effective knowledge transfer, 5 support the environmental biomonitoring sector to achieve the required changes, 6 steer the process and harmonise efforts at the European level. This paper provides an overview of the forum discussions and the common European views that have emerged from them, while reflecting the diversity of situations in different countries. It highlights important actions required for a successful implementation of DNA based biomonitoring of aquatic ecosystems by 2030. 6 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85137246592 origin=inward Articolo in rivista Pensoft 2534 9708 Metabarcoding and Metagenomics Metabarcoding and Metagenomics Metabarcoding and Metagenomics Metabarcoding Metagenom MBMG Metabarcoding Metagenomics stefania.erba ERBA STEFANIA |
