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DatoValore
TitleFull-scale mesophilic anaerobic sludge digester: microbial community dynamics from start-up to steady state
AbstractThis study aimed at investigating bacterial and archaeal dynamics in a full-scale mesophilic digester for the treatment of activated sludge, during the start-up phase up to steady state operation. Microorganisms developed in the reactor were investigated using the high throughput 16S rRNA gene sequencing. The results showed that most of the reads were affiliated with hydrolitic and fermentative bacteria, mainly belonging to Bacteroidetes phylum, which increased up to 22% of the total sequences over time. Moreover, methanogenic community was represented mainly by acetoclastic microorganisms, namely Methanosaeta. Real Time PCR revealed an enrichment of bacteria and methanogens once the steady state was reached. Furthermore, activated sludge was studied to evaluate the microbial core selected by the operating conditions of the wastewater treatment plant.
Source9th international symposium on energy from biomass and waste, Venice (Italy), 21-23/11/2022
KeywordsAnaerobic DigestionFull Scale PlantWaste Activated SludgeMicrobial Population Dynamics
Year2022
TypeContributo in atti di convegno
AuthorsB. Tonanzi, S. Crognale, A. Gianico, S. Della Sala, P. Miana, M.C. Zaccone and S. Rossetti
Text475006 2022 Anaerobic Digestion Full Scale Plant Waste Activated Sludge Microbial Population Dynamics Full scale mesophilic anaerobic sludge digester microbial community dynamics from start up to steady state B. Tonanzi, S. Crognale, A. Gianico, S. Della Sala, P. Miana, M.C. Zaccone and S. Rossetti National Research Council of Italy Water Research Institute CNR IRSA, Area della Ricerca RM1, 00015 Monterotondo, Rome, Italy. Veritas S.p.a., 30135 Venezia, Italy. This study aimed at investigating bacterial and archaeal dynamics in a full scale mesophilic digester for the treatment of activated sludge, during the start up phase up to steady state operation. Microorganisms developed in the reactor were investigated using the high throughput 16S rRNA gene sequencing. The results showed that most of the reads were affiliated with hydrolitic and fermentative bacteria, mainly belonging to Bacteroidetes phylum, which increased up to 22% of the total sequences over time. Moreover, methanogenic community was represented mainly by acetoclastic microorganisms, namely Methanosaeta. Real Time PCR revealed an enrichment of bacteria and methanogens once the steady state was reached. Furthermore, activated sludge was studied to evaluate the microbial core selected by the operating conditions of the wastewater treatment plant. Proceedings of the 9th international symposium on energy from biomass and waste, Venice Italy , 21 23 November 2022 Cisa publisher 9788862650298 Published version 9th international symposium on energy from biomass and waste Venice Italy 21 23/11/2022 Internazionale Contributo Full scale mesophilic anaerobic sludge digester microbial community dynamics from start up to steady state Conference paper 2022 Venice_2129_Tonanzi.pdf Contributo in atti di convegno TONANZI BARBARA simona.rossetti ROSSETTI SIMONA andrea.gianico GIANICO ANDREA simona.crognale CROGNALE SIMONA