| Title | High Purity Grade Phycocyanin Recovery by Decupling Cell Lysis from the Pigment Extraction: an Innovative Approach |
| Abstract | Phycocyanin, a phycobiliprotein, is one of the few natural blue pigments available as food colourant, and it is largely used in food industry. We have devised an innovative two-step extraction process which allowed to obtain bright blue phycocyanin crude extracts with high purity grade P (within 2.5 and 3.5) directly from fresh biomass of Arthrospira platensis Gomont 1892 (commonly named Spirulina). We found out and for the first time exploited ammonium sulphate capability to minimize the release of water soluble phycobiliproteins in aqueous medium during ultrasound-assisted cell lysis/purification phase. The conventional sequence which is, extraction followed by purification, was reversed. The extraction phase was decoupled from biomass cell lysis. Cell lysis, accomplished by ultrasonication in ammonium sulphate solution, was merged with purification in a single step, before the pigment extraction/recovering phase. The process was entirely carried out in aqueous solutions. No downstream purification was required to obtain products suitable for the most common phycocyanin applications (i.e. foods, nutraceuticals). Production time, hours instead of days, was reduced to the advantage of the product quality. The process has the great advantages of (1) direct use of extracting solutions that cannot be used in the ordinary ultrasound-assisted extraction of phycocyanin (because of the extensive simultaneous extraction of contaminant molecules), (2) gain of high commercial value phycocyanin due to the elevated purity grade and (3) direct production of highly concentrated bright blue pigment crude extracts (up to about 5 mg mL) immediately in hand to the market. Graphical Abstract: [Figure not available: see fulltext.] |
| Source | Food and bioprocess technology (Print) |
| Keywords | PhycocyaninHigh purity gradeInnovative ultrasound-assisted extractionAmmonium sulphateArthrospira platensisReady-to-market |
| Journal | Food and bioprocess technology (Print) |
| Editor | Springer Science + Business Media, New York, NY, Stati Uniti d'America |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1007/s11947-022-02926-w |
| Authors | Lauceri R.; Cavone C.; Chini Zittelli G.; Kamburska L.; Musazzi S.; Torzillo G. |
| Text | 472937 2022 10.1007/s11947 022 02926 w Scopus 2 s2.0 85140854039 ISI Web of Science WOS 000875505800001 Phycocyanin High purity grade Innovative ultrasound assisted extraction Ammonium sulphate Arthrospira platensis Ready to market High Purity Grade Phycocyanin Recovery by Decupling Cell Lysis from the Pigment Extraction an Innovative Approach Lauceri R.; Cavone C.; Chini Zittelli G.; Kamburska L.; Musazzi S.; Torzillo G. Istituto di Ricerca Sulle Acque, Consiglio Nazionale delle Ricerche, Sede di Verbania, Largo Tonolli 50, 28922 Verbania, Italy; Dipartimento Bioscienze e Territorio, Universita del Molise, C.da Fonte Lappone, 86090 Pesche, Italy; Istituto per la Bioeconomia, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, Sesto Fiorentino, Firenze I 50019, Italy; Centro de Investigacion en Ciencias Del Mar y Limnolog a, Universidad de Costa Rica, San Pedro, San Jose 2060, Costa Rica Phycocyanin, a phycobiliprotein, is one of the few natural blue pigments available as food colourant, and it is largely used in food industry. We have devised an innovative two step extraction process which allowed to obtain bright blue phycocyanin crude extracts with high purity grade P within 2.5 and 3.5 directly from fresh biomass of Arthrospira platensis Gomont 1892 commonly named Spirulina . We found out and for the first time exploited ammonium sulphate capability to minimize the release of water soluble phycobiliproteins in aqueous medium during ultrasound assisted cell lysis/purification phase. The conventional sequence which is, extraction followed by purification, was reversed. The extraction phase was decoupled from biomass cell lysis. Cell lysis, accomplished by ultrasonication in ammonium sulphate solution, was merged with purification in a single step, before the pigment extraction/recovering phase. The process was entirely carried out in aqueous solutions. No downstream purification was required to obtain products suitable for the most common phycocyanin applications i.e. foods, nutraceuticals . Production time, hours instead of days, was reduced to the advantage of the product quality. The process has the great advantages of 1 direct use of extracting solutions that cannot be used in the ordinary ultrasound assisted extraction of phycocyanin because of the extensive simultaneous extraction of contaminant molecules , 2 gain of high commercial value phycocyanin due to the elevated purity grade and 3 direct production of highly concentrated bright blue pigment crude extracts up to about 5 mg mL immediately in hand to the market. Graphical Abstract Figure not available see fulltext. Published version https //link.springer.com/article/10.1007/s11947 022 02926 w 21/10/2022 High Purity Grade Phycocyanin Recovery by Decupling Cell Lysis from the Pigment Extraction an Innovative Approach Articolo in rivista 2022 Lauceri High Purity Grade Phycocyanin Recovery by Decupling Cell Lysis from the Pigment Extraction_an Innovative Approach.pdf Articolo in rivista Springer Science Business Media 1935 5130 Food and bioprocess technology Print Food and bioprocess technology Print Food bioproc. tech. Print Food and bioprocess technology. Print giuseppe.torzillo TORZILLO GIUSEPPE CAVONE CRISTINA rosaria.lauceri LAUCERI ROSARIA graziella.chinizittelli CHINI ZITTELLI GRAZIELLA lyudmilatodorova.kamburska KAMBURSKA LYUDMILA TODOROVA simona.musazzi MUSAZZI SIMONA |
