| Abstract | Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose-Binding Lectin (SsGBL) was able to agglutinate bacteria and to preferentially recognize Gram-negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram-positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions. |
| Text | 472721 2019 Scopus 2 s2.0 85065887935 mucus; hemagglutinin; bacteria; galactose binding lectin; S. spallanzanii Sabella spallanzanii mucus contain a galactose binding lectin able to agglutinate bacteria. Purification and characterization Cammarata M.; Benenati G.; Dara M.; Parisi M.G.; Piazzese D.; Falco F.; Stabili L. Department of Heart and Marine Science DISTEM, Marine Immunobiology Laboratory, University of Palermo, Department of Heart and Marine Science DISTEM, Marine Immunobiology Laboratory, University of Palermo, Italy, , Italy; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies STEBICEF, University of Palermo, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies STEBICEF, University of Palermo, Italy, , Italy; Istituto per le Risorse Biologiche e le Biotecnologie Marine IRBM , Units of Capo Granitola, Istituto per le Risorse Biologiche e le Biotecnologie Marine IRBM , Units of Capo Granitola Italy, , Italy; Istituto per l Ambiente Marino Costiero, U.O.S. di Taranto, CNR, Taranto, Istituto per l Ambiente Marino Costiero, U.O.S. di Taranto, CNR, Taranto, Italy, , Italy; Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy, , Italy Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose Binding Lectin SsGBL was able to agglutinate bacteria and to preferentially recognize Gram negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions. 16 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85065887935 origin=inward Articolo in rivista Enzo Ottaviani 1824 307X Invertebrate survival journal Invertebrate survival journal Invertebrate surviv. j. Invertebrate survival journal. ISJ loredana.stabili STABILI LOREDANA |
