Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Contamination of soil and groundwater by nitrate: a new methodologic approach to the identification of source through biomolecular analyses |
| Abstract | Currently, there is not yet defined a microbiologically-based method for identifying sources of nitrate contamination. This work aims at identifying a standard methodology based on biomolecular analyses, which can reveal the origin of the contamination. In the DEMETRA DSS project, techniques and activities for monitoring agricultural soils and groundwater were developed for the identification of eventual correlations between the soil and the groundwater and for bringing up possible nitrate contamination from agricultural source. A specific method of sampling and analysis, both chemical and microbiological, of the two matrices has been fully defined, in order to discriminate whether any nitrate present in the water originates from an incorrect agricultural practice or comes from upstream areas. Starting from nitrate pollution prone areas and the characterization of the territory, 77 sites, located throughout the Apulian territory in six monitoring sub-areas, were identified and sampled for chemical analyses, relative to the nitrogen line, and biomolecular analyses. Specific REAL TIME-PCR protocols were developed and applied to water and soil samples, and the analysis focused on microorganisms involved in nitrification and denitrification activities. The application of real time techniques allowed the identification and quantification of specific bacterial species linked to the different matrices at the different sites, thus making it possible to determine the microbial activity linked to the nitrogen cycle |
| Source | 5th EWaS International Conference, Napoli, 12-15/07/2022 |
| Keywords | soilshallow groundwaternitrate pollutionbiomolecular technique |
| Year | 2022 |
| Type | Contributo in atti di convegno |
| Authors | Angelantonio Calabrese, Massimo Blonda, Laura Mandrelli, Erika Loi, Emanuele Barca and Giuseppe Pappagallo |
| Text | 469227 2022 soil shallow groundwater nitrate pollution biomolecular technique Contamination of soil and groundwater by nitrate a new methodologic approach to the identification of source through biomolecular analyses Angelantonio Calabrese, Massimo Blonda, Laura Mandrelli, Erika Loi, Emanuele Barca and Giuseppe Pappagallo Consiglio Nazionale delle Ricerche Istituto di Ricerca Sulle Acque, Bari, 70132, Italy Currently, there is not yet defined a microbiologically based method for identifying sources of nitrate contamination. This work aims at identifying a standard methodology based on biomolecular analyses, which can reveal the origin of the contamination. In the DEMETRA DSS project, techniques and activities for monitoring agricultural soils and groundwater were developed for the identification of eventual correlations between the soil and the groundwater and for bringing up possible nitrate contamination from agricultural source. A specific method of sampling and analysis, both chemical and microbiological, of the two matrices has been fully defined, in order to discriminate whether any nitrate present in the water originates from an incorrect agricultural practice or comes from upstream areas. Starting from nitrate pollution prone areas and the characterization of the territory, 77 sites, located throughout the Apulian territory in six monitoring sub areas, were identified and sampled for chemical analyses, relative to the nitrogen line, and biomolecular analyses. Specific REAL TIME PCR protocols were developed and applied to water and soil samples, and the analysis focused on microorganisms involved in nitrification and denitrification activities. The application of real time techniques allowed the identification and quantification of specific bacterial species linked to the different matrices at the different sites, thus making it possible to determine the microbial activity linked to the nitrogen cycle Postprint 5th EWaS International Conference Napoli 12 15/07/2022 Internazionale Contributo Contamination of soil and groundwater by nitrate a new methodologic approach to the identification of source through biomolecular analyses EWaS_ DEMETRA DSS Biomolecolare.pdf Contributo in atti di convegno lauramandrelli MANDRELLI LAURA erikaloi LOI ERIKA emanuele.barca BARCA EMANUELE giuseppe.pappagallo PAPPAGALLO GIUSEPPE angelantonio.calabrese CALABRESE ANGELANTONIO massimo.blonda BLONDA MASSIMO DTA.AD002.587.001 iDEntificazione MEtagenomica delle fonTi di contaminazione da nitRAti e Sistemi di Supporto Decisionale DSS per una gestione agronomica sostenibile |