| Text | 469181 2021 10.1007/978 1 0716 1115 9_4 Scopus 2 s2.0 85101471061 FISH environmental samples unculturable bacteria FISH in Suspension or in Adherent Cells Di Pippo F.; Queiros D.; Pereira J.; Lemos P.C.; Serafim L.S.; Rossetti S. Water Research Institute C.N.R., Monterotondo, Water Research Institute C.N.R., Monterotondo, Italy, , Italy; CICECO Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, CICECO Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal, , , Portugal; CICECO Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, CICECO Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal, , , Portugal; LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, Portugal, , , Portugal; LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, Portugal, , , Portugal Fluorescence in situ hybridization FISH enables the detection and enumeration of microorganisms in a diversity of samples. Short length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples. Published version http //www.scopus.com/record/display.url eid=2 s2.0 85101471061 origin=inward Capitolo Contributo in volume simona.rossetti ROSSETTI SIMONA francesca.dipippo DI PIPPO FRANCESCA |
