Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Insights into structure and functioning of a soil microbial community amended with cattle manure digestate and sulfamethoxazole |
| Abstract | Purpose: The present work aimed to fill some knowledge gaps on the effects on non-target natural soil microbial communities of the sulfamethoxazole (SMX) antibiotic potentially found in cattle manure digestate. Both soil prokaryotic and fungal community under different conditions were analyzed using molecular ecology methods. Methods: A previous microcosm experiment with a soil amended with a cattle manure digestate (3% dry mass) and spiked with SMX (20 mg/kg soil) was used for this in-depth study. Microbial live cell abundances were assessed by direct epifluorescence microscope methods. The microbial community structures were studied by DNA extraction and amplification using 16S rRNA primers targeting the V3-V4 region (for prokaryotes) and ITS1 (for fungi); Alpha-diversity indices (Chao1, Shannon and Evenness) were also estimated. Moreover, a prediction functional analysis was performed on prokaryotic 16S rRNA amplicon data with PICRUSt2 in order to predict possible variations in some microbial functioning. Results: Adding digestate to soil promoted both microbial abundance and some bacterial groups. The co-presence of SMX initially lowered these positive effects and decreased diversity. However, at day 61, the antibiotic was almost all removed and the microbial abundance and prokaryotic and fungal diversity showed increased values. Conclusion: The antibiotic detrimental effects on prokaryotic cells were transient and some resistant bacteria (e.g., Sphingomonas, Skermanella), presumably introduced into the soil with the digestate, were able to remove SMX. Moreover, the digestate long-term effect was to favor some fungal groups such as Basidiomycota. The presence of the genus Trichoderma (Ascomycota), able to transform antibiotics, does not exclude also as possible involvement of this fungus in SMX removal. |
| Source | Journal of soils and sediments (Print) |
| Keywords | Sulphonamide antibioticsMicrobial nitrogen metabolismMicrobial oxidative stressProkaryotic and fungal community |
| Journal | Journal of soils and sediments (Print) |
| Editor | Springer, Heidelberg ;, Germania |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1007/s11368-022-03222-y |
| Authors | Garbini G.L.; Grenni P.; Rauseo J.; Patrolecco L.; Pescatore T.; Spataro F.; Barra Caracciolo A. |
| Text | 468920 2022 10.1007/s11368 022 03222 y Scopus 2 s2.0 85130180369 Sulphonamide antibiotics Microbial nitrogen metabolism Microbial oxidative stress Prokaryotic and fungal community Insights into structure and functioning of a soil microbial community amended with cattle manure digestate and sulfamethoxazole Garbini G.L.; Grenni P.; Rauseo J.; Patrolecco L.; Pescatore T.; Spataro F.; Barra Caracciolo A. Garbini G.L.; Grenni P.; Barra Caracciolo A. CNR IRSA Rauseo J.; Patrolecco L.; Pescatore T.; Spataro F. CNR ISP Purpose The present work aimed to fill some knowledge gaps on the effects on non target natural soil microbial communities of the sulfamethoxazole SMX antibiotic potentially found in cattle manure digestate. Both soil prokaryotic and fungal community under different conditions were analyzed using molecular ecology methods. Methods A previous microcosm experiment with a soil amended with a cattle manure digestate 3% dry mass and spiked with SMX 20 mg/kg soil was used for this in depth study. Microbial live cell abundances were assessed by direct epifluorescence microscope methods. The microbial community structures were studied by DNA extraction and amplification using 16S rRNA primers targeting the V3 V4 region for prokaryotes and ITS1 for fungi ; Alpha diversity indices Chao1, Shannon and Evenness were also estimated. Moreover, a prediction functional analysis was performed on prokaryotic 16S rRNA amplicon data with PICRUSt2 in order to predict possible variations in some microbial functioning. Results Adding digestate to soil promoted both microbial abundance and some bacterial groups. The co presence of SMX initially lowered these positive effects and decreased diversity. However, at day 61, the antibiotic was almost all removed and the microbial abundance and prokaryotic and fungal diversity showed increased values. Conclusion The antibiotic detrimental effects on prokaryotic cells were transient and some resistant bacteria e.g., Sphingomonas, Skermanella , presumably introduced into the soil with the digestate, were able to remove SMX. Moreover, the digestate long term effect was to favor some fungal groups such as Basidiomycota. The presence of the genus Trichoderma Ascomycota , able to transform antibiotics, does not exclude also as possible involvement of this fungus in SMX removal. Published version http //www.scopus.com/record/display.url eid=2 s2.0 85130180369 origin=inward articolo puBBLICATO Garbini2022_Article_InsightsIntoStructureAndFuncti.pdf Articolo in rivista Springer 1439 0108 Journal of soils and sediments Print Journal of soils and sediments Print J. soils sediments Print Journal of soils and sediments. Print JSS Landsberg. Print Print J soils sediments Print Print Journal of soils sediments Print JSS Berlin. Print Print JSSS Print Print Journal of soil and sediments Print tanitapescatore PESCATORE TANITA GARBINI GIAN LUIGI luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA francesca.spataro SPATARO FRANCESCA |