| Title | Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing |
| Abstract | Delayed wound healing and chronic skin lesions represent a major health problem. Over the past years, growth factors mediated by platelet-rich plasma (PRP) and cell-based therapies were developed as effective and affordable treatment able to improve wound healing capacity. We have advanced existing concepts to develop a highly efficient high-throughput protocol with proven application for the isolation of PRP and pro-angiogenic cells (Angio(PRP)). This protocol outlines the effectiveness of Angio(PRP) in promoting the critical healing process including wound closure, re-epithelialization, granulation tissue growth, and blood vessel regeneration. We coupled this effect with normalization of mechanical properties of rescued mouse wounds, which is sustained by a correct arrangement of elastin and collagen fibers. Proteomic analysis of treated wounds demonstrated a fingerprint of Angio(PRP) based on the up-regulation of detoxification pathway of glutathione metabolism, correlated to a decrease in inflammatory response. Overall, these results have enabled us to provide a framework for how Angio(PRP) supports wound healing, opening avenues for further clinical advances. |
| Source | Cellular and molecular life sciences (Print. ed.) 79 (5) |
| Keywords | EpithelializationPRPAngiogenic potentialSkin remodelingProteomics |
| Journal | Cellular and molecular life sciences (Print. ed.) |
| Editor | Springer Basel AG, Basel, Svizzera |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00018-022-04284-4 |
| Authors | Erratico, Silvia; Belicchi, Marzia; Meregalli, Mirella; Di Silvestre, Dario; Tripodi, Luana; De Palma, Antonella; Jones, Rebecca; Ferrari, Emanuele; Porretti, Laura; Trombetta, Elena; Merlo, Giorgio R.; Mauri, Pierluigi; Torrente, Yvan |
| Text | 468215 2022 10.1007/s00018 022 04284 4 ISI Web of Science WOS 000787796500002 PubMed 35474498 Epithelialization PRP Angiogenic potential Skin remodeling Proteomics Effective high throughput isolation of enriched platelets and circulating pro angiogenic cells to accelerate skin wound healing Erratico, Silvia; Belicchi, Marzia; Meregalli, Mirella; Di Silvestre, Dario; Tripodi, Luana; De Palma, Antonella; Jones, Rebecca; Ferrari, Emanuele; Porretti, Laura; Trombetta, Elena; Merlo, Giorgio R.; Mauri, Pierluigi; Torrente, Yvan Novystem Spa; Univ Milan; Natl Res Council ITB CNR; Univ Torino; Fdn IRCCS Ca Granda Osped Maggiore Policlin Delayed wound healing and chronic skin lesions represent a major health problem. Over the past years, growth factors mediated by platelet rich plasma PRP and cell based therapies were developed as effective and affordable treatment able to improve wound healing capacity. We have advanced existing concepts to develop a highly efficient high throughput protocol with proven application for the isolation of PRP and pro angiogenic cells Angio PRP . This protocol outlines the effectiveness of Angio PRP in promoting the critical healing process including wound closure, re epithelialization, granulation tissue growth, and blood vessel regeneration. We coupled this effect with normalization of mechanical properties of rescued mouse wounds, which is sustained by a correct arrangement of elastin and collagen fibers. Proteomic analysis of treated wounds demonstrated a fingerprint of Angio PRP based on the up regulation of detoxification pathway of glutathione metabolism, correlated to a decrease in inflammatory response. Overall, these results have enabled us to provide a framework for how Angio PRP supports wound healing, opening avenues for further clinical advances. 79 Published version Articolo in rivista Springer Basel AG 1420 682X Cellular and molecular life sciences Print. ed. Cellular and molecular life sciences Print. ed. Cell. mol. life sci. Print. ed. Cellular and molecular life sciences Print. ed. CMLS Print. ed. antonella.depalma DE PALMA ANTONELLA dario.disilvestre DI SILVESTRE DARIO emanuele.ferrari FERRARI EMANUELE pietroluigi.mauri MAURI PIETRO LUIGI |
