Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Bioactive Potential of Two Marine Picocyanobacteria Belonging to Cyanobium and Synechococcus Genera |
| Abstract | Coccoid cyanobacteria produce a great variety of secondary metabolites, which may have useful properties, such as antibacterial, antiviral, anticoagulant or anticancer activities. These cyanobacterial metabolites have high ecological significance, and they could be considered responsible for the widespread occurrence of these microorganisms. Considering the great benefit derived from the identification of competent cyanobacteria for the extraction of bioactive compounds, two strains of picocyanobacteria (coccoid cyanobacteria < 3 mu m) (Cyanobium sp. ITAC108 and Synechococcus sp. ITAC107) isolated from the Mediterranean sponge Petrosia ficiformis were analyzed. The biological effects of organic and aqueous extracts from these picocyanobacteria toward the nauplii of Artemia salina, sea urchin embryos and human cancer lines (HeLa cells) were evaluated. Methanolic and aqueous extracts from the two strains strongly inhibited larval development; on the contrary, in ethyl acetate and hexane extracts, the percentage of anomalous embryos was low. Moreover, all the extracts of the two strains inhibited HeLa cell proliferation, but methanol extracts exerted the highest activity. Gas chromatography-mass spectrometry analysis evidenced for the first time the presence of beta-N-methylamino-l-alanine and microcystin in these picocyanobacteria. The strong cytotoxic activity observed for aqueous and methanolic extracts of these two cyanobacteria laid the foundation for the production of bioactive compounds of pharmacological interest. |
| Source | Microorganisms 9 (10) |
| Keywords | picocyanobacteria toxicityCyanobiumSynechococcusArtemia salinasea urchin embryosHeLa cell linemicrocystinBMAA |
| Journal | Microorganisms |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.3390/microorganisms9102048 |
| Authors | Pagliara P.; De Benedetto G.E.; Francavilla M.; Barca A.; Caroppo C. |
| Text | 466328 2021 10.3390/microorganisms9102048 ISI Web of Science WOS 000713177200001 picocyanobacteria toxicity Cyanobium Synechococcus Artemia salina sea urchin embryos HeLa cell line microcystin BMAA Bioactive Potential of Two Marine Picocyanobacteria Belonging to Cyanobium and Synechococcus Genera Pagliara P.; De Benedetto G.E.; Francavilla M.; Barca A.; Caroppo C. Universita del Salento; Universita del Salento; CNR ISPC, Lecce, Italy; Universita di Foggia; CNR IRSA, Taranto, Italy Coccoid cyanobacteria produce a great variety of secondary metabolites, which may have useful properties, such as antibacterial, antiviral, anticoagulant or anticancer activities. These cyanobacterial metabolites have high ecological significance, and they could be considered responsible for the widespread occurrence of these microorganisms. Considering the great benefit derived from the identification of competent cyanobacteria for the extraction of bioactive compounds, two strains of picocyanobacteria coccoid cyanobacteria < 3 mu m Cyanobium sp. ITAC108 and Synechococcus sp. ITAC107 isolated from the Mediterranean sponge Petrosia ficiformis were analyzed. The biological effects of organic and aqueous extracts from these picocyanobacteria toward the nauplii of Artemia salina, sea urchin embryos and human cancer lines HeLa cells were evaluated. Methanolic and aqueous extracts from the two strains strongly inhibited larval development; on the contrary, in ethyl acetate and hexane extracts, the percentage of anomalous embryos was low. Moreover, all the extracts of the two strains inhibited HeLa cell proliferation, but methanol extracts exerted the highest activity. Gas chromatography mass spectrometry analysis evidenced for the first time the presence of beta N methylamino l alanine and microcystin in these picocyanobacteria. The strong cytotoxic activity observed for aqueous and methanolic extracts of these two cyanobacteria laid the foundation for the production of bioactive compounds of pharmacological interest. 9 Published version https //doi.org/10.3390/microorganisms9102048 28/09/2021 Bioactive Potential of Two Marine Picocyanobacteria Belonging to Cyanobium and Synechococcus Genera 2021_466328_published.pdf Articolo in rivista Molecular Diversity Preservation International 2076 2607 Microorganisms Microorganisms DE BENEDETTO GIUSEPPE EGIDIO carmela.caroppo CAROPPO CARMELA |