Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Effects of Sulfamethoxazole on Growth and Antibiotic Resistance of A Natural Microbial Community |
| Abstract | Diffuse environmental antibiotic and antibiotic resistance gene contamination is increasing human and animal exposure to these emerging compounds with a consequent risk of reduction in antibiotic effectiveness. The present work investigated the effect of the antibiotic sulfamethoxazole (SMX) on growth and antibiotic resistance genes of a microbial community collected from an anaerobic digestion plant fed with cattle manure. Digestate samples were used as inoculum for concentration-dependent experiments using SMX at various concentrations. The antibiotic concentrations affecting the mixed microbial community in terms of growth and spread of resistant genes (sul1, sul2) were investigated through OD (Optical Density) measures and qPCR assays. Moreover, SMX biodegradation was assessed by LC-MS/MS analysis. The overall results showed that SMX concentrations in the range of those found in the environment did not affect the microbial community growth and did not select for antibiotic-resistant gene (ARG) maintenance or spread. Furthermore, the microorganisms tested were able to degrade SMX in only 24 h. This study confirms the complexity of antibiotic resistance spread in real matrices where different microorganisms coexist and suggests that antibiotic biodegradation needs to be included for fully understanding the resistance phenomena among bacteria. |
| Source | Water (Basel) 13 (9) |
| Keywords | intI1sul1sul2antibiotic degradationMSC |
| Journal | Water (Basel) |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.3390/w13091262 |
| Authors | Rauseo, Jasmin; Barra Caracciolo, Anna; Spataro, Francesca; Visca, Andrea; Ademollo, Nicoletta; Pescatore, Tanita; Grenni, Paola; Patrolecco, Luisa |
| Text | 465877 2021 10.3390/w13091262 ISI Web of Science WOS 000650886900001 intI1 sul1 sul2 antibiotic degradation MSC Effects of Sulfamethoxazole on Growth and Antibiotic Resistance of A Natural Microbial Community Rauseo, Jasmin; Barra Caracciolo, Anna; Spataro, Francesca; Visca, Andrea; Ademollo, Nicoletta; Pescatore, Tanita; Grenni, Paola; Patrolecco, Luisa ISP CNR; IRSA CNR Diffuse environmental antibiotic and antibiotic resistance gene contamination is increasing human and animal exposure to these emerging compounds with a consequent risk of reduction in antibiotic effectiveness. The present work investigated the effect of the antibiotic sulfamethoxazole SMX on growth and antibiotic resistance genes of a microbial community collected from an anaerobic digestion plant fed with cattle manure. Digestate samples were used as inoculum for concentration dependent experiments using SMX at various concentrations. The antibiotic concentrations affecting the mixed microbial community in terms of growth and spread of resistant genes sul1, sul2 were investigated through OD Optical Density measures and qPCR assays. Moreover, SMX biodegradation was assessed by LC MS/MS analysis. The overall results showed that SMX concentrations in the range of those found in the environment did not affect the microbial community growth and did not select for antibiotic resistant gene ARG maintenance or spread. Furthermore, the microorganisms tested were able to degrade SMX in only 24 h. This study confirms the complexity of antibiotic resistance spread in real matrices where different microorganisms coexist and suggests that antibiotic biodegradation needs to be included for fully understanding the resistance phenomena among bacteria. 13 Published version 29/04/2021 Articolo pubblicato pdf water 13 01262.pdf Articolo in rivista Molecular Diversity Preservation International 2073 4441 Water Basel Water Basel Water Basel Water. Basel VISCA ANDREA tanitapescatore PESCATORE TANITA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA francesca.spataro SPATARO FRANCESCA jasmin.rauseo RAUSEO JASMIN |