| Title | Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments |
| Abstract | Wastewater treatment plants (WWTPs) are among the main hotspots of antibiotic resistance genes (ARGs) in the environment. Previously, we demonstrated that, by increasing anthropogenic pollution, the antibiotic resistome persisted in the microbial community of rivers and lakes, independently by changes in community composition. In this study, we reanalysed the data to test for the relation of metal resistance genes (MRGs), plasmids, and integrons to the persistence of the antibiotic resistome. The experiment consisted in replicated co-cultures of riverine or lacustrine microbial communities and WWTP effluents in different proportions. Samples before (T0) and after a short period of incubation (TF) were collected and community metagenomic data were obtained by shotgun sequencing. The data were processed to annotate MRGs, plasmids, and integrases. The integrases stabilized in the aquatic environment following the degree of contamination with effluent water (in particular in one site), whereas MRGs and plasmids showed stochastic trajectories. These results confirm the potential correlation between integrons and anthropogenic pollution, and the reliability of intI1 as a pollution marker. Only in one site MRGs, plasmids, and ARGs were correlated, highlighting their partial contribution to the persistence of ARGs in surface waters. |
| Source | Environmental pollution (1987) 297 (118774) |
| Keywords | integronplasmidome |
| Journal | Environmental pollution (1987) |
| Editor | Elsevier Applied Science Publishers, Barking, Regno Unito |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.envpol.2021.118774 |
| Authors | Andrea Di Cesare, Raffaella Sabatino, Ying Yang, Diego Brambilla, Pu Li, Diego Fontaneto, Ester M. Eckert, Gianluca Corno |
| Text | 462170 2022 10.1016/j.envpol.2021.118774 integron plasmidome Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments Andrea Di Cesare, Raffaella Sabatino, Ying Yang, Diego Brambilla, Pu Li, Diego Fontaneto, Ester M. Eckert, Gianluca Corno Water Research Institute IRSA MEG Molecular Ecology Group, CNR National Research Council of Italy, Largo Tonolli 50, 28922, Verbania, Italy School of Marine Sciences, Sun Yat sen University, 519082, Zhuhai, China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai , 519082, Zhuhai, China Pearl River Estuary Marine Ecosystem Research Station, Ministry of Education, Zhuhai, 519082, China Wastewater treatment plants WWTPs are among the main hotspots of antibiotic resistance genes ARGs in the environment. Previously, we demonstrated that, by increasing anthropogenic pollution, the antibiotic resistome persisted in the microbial community of rivers and lakes, independently by changes in community composition. In this study, we reanalysed the data to test for the relation of metal resistance genes MRGs , plasmids, and integrons to the persistence of the antibiotic resistome. The experiment consisted in replicated co cultures of riverine or lacustrine microbial communities and WWTP effluents in different proportions. Samples before T0 and after a short period of incubation TF were collected and community metagenomic data were obtained by shotgun sequencing. The data were processed to annotate MRGs, plasmids, and integrases. The integrases stabilized in the aquatic environment following the degree of contamination with effluent water in particular in one site , whereas MRGs and plasmids showed stochastic trajectories. These results confirm the potential correlation between integrons and anthropogenic pollution, and the reliability of intI1 as a pollution marker. Only in one site MRGs, plasmids, and ARGs were correlated, highlighting their partial contribution to the persistence of ARGs in surface waters. 297 Published version https //www.sciencedirect.com/science/article/pii/S0269749121023563 via%3Dihub dc Di Cesare et al 2022.pdf Articolo in rivista Elsevier Applied Science Publishers 0269 7491 Environmental pollution 1987 Environmental pollution 1987 Environ. pollut. 1987 Environmental pollution 1987 SABATINO RAFFAELLA BRAMBILLA DIEGO gianluca.corno CORNO GIANLUCA diego.fontaneto FONTANETO DIEGO andrea.dicesare DI CESARE ANDREA estermaria.eckert ECKERT ESTER MARIA |
