| Title | Antibiotics and antibiotic resistance genes in anaerobic digesters and predicted concentrations in agroecosystems |
| Abstract | In recent decades, the innovative practice of management and valorization of agrozootechnical waste as energy through anaerobic digestion (AD) has been rapidly growing. However, whether applying digestate to soil as biofertilizer can be a source of antibiotics (ABs) and antibiotic resistance genes (ARGs) has not been fully investigated so far. In this work the ARGs responsible for sulfamethoxazole (SMX) resistance (sul1, sul2), ciprofloxacin (CIP) resistance (qnrS, qepA, aac-(6 ')-Ib-cr) and the mobile genetic element intl1, together with the concentrations of the antibiotics SMX and CIP, were measured in several anaerobic digesters located in Central Italy. Based on these results, the concentrations of antibiotics and ARGs which can potentially reach soil through amendment with digestate were also estimated. The highest CIP and SMX concentrations were found during winter and spring in anaerobic digesters. The highest ARG abundances were found for the aac-(6')-Ib-cr and sul2 genes. The overall results showed that application of digestate to soil does not exclude AB contamination and spread of ARGs in agroecosystems, especially in the case of ciprofloxacin, owing to its high intrinsic persistence. |
| Source | Journal of environmental management 301, pp. 113891 |
| Keywords | SulfamethoxazoleCiprofloxacinCattle manureDigestateZootechnical waste |
| Journal | Journal of environmental management |
| Editor | Academic Press., New York, Regno Unito |
| Year | 2022 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.jenvman.2021.113891 |
| Authors | Visca, Andrea; Rauseo, Jasmin; Spataro, Francesca; Patrolecco, Luisa; Grenni, Paola; Massini, Giulia; Miritana, Valentina Mazzurco; Barra Caracciolo, Anna |
| Text | 461945 2022 10.1016/j.jenvman.2021.113891 ISI Web of Science WOS 000711337500009 Sulfamethoxazole Ciprofloxacin Cattle manure Digestate Zootechnical waste Antibiotics and antibiotic resistance genes in anaerobic digesters and predicted concentrations in agroecosystems Visca, Andrea; Rauseo, Jasmin; Spataro, Francesca; Patrolecco, Luisa; Grenni, Paola; Massini, Giulia; Miritana, Valentina Mazzurco; Barra Caracciolo, Anna Natl Res Council IRSA CNR; Natl Res Council ISP CNR; Italian Natl Agcy New Technol Energy Sustainabl In recent decades, the innovative practice of management and valorization of agrozootechnical waste as energy through anaerobic digestion AD has been rapidly growing. However, whether applying digestate to soil as biofertilizer can be a source of antibiotics ABs and antibiotic resistance genes ARGs has not been fully investigated so far. In this work the ARGs responsible for sulfamethoxazole SMX resistance sul1, sul2 , ciprofloxacin CIP resistance qnrS, qepA, aac 6 Ib cr and the mobile genetic element intl1, together with the concentrations of the antibiotics SMX and CIP, were measured in several anaerobic digesters located in Central Italy. Based on these results, the concentrations of antibiotics and ARGs which can potentially reach soil through amendment with digestate were also estimated. The highest CIP and SMX concentrations were found during winter and spring in anaerobic digesters. The highest ARG abundances were found for the aac 6 Ib cr and sul2 genes. The overall results showed that application of digestate to soil does not exclude AB contamination and spread of ARGs in agroecosystems, especially in the case of ciprofloxacin, owing to its high intrinsic persistence. 301 Published version JEM_2021 International Paper JEM_2021.pdf Articolo in rivista Academic Press. 0301 4797 Journal of environmental management Journal of environmental management J. environ. manag. VISCA ANDREA MAZZURCO MIRITANA VALENTINA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA francesca.spataro SPATARO FRANCESCA jasmin.rauseo RAUSEO JASMIN |
