| Abstract | Is it possible to improve the efficiency of bioremediation technologies? The use of mixed cultures of bacteria and fungi inoculated at the rhizosphere level could promote the growth of the associated hyperaccumulating plant species and increase the absorption of metals in polluted soils, broadening new horizons on bioremediation purposes. This work investigates interactions between Ni-tolerant plant growth-promoting bacteria and fungi (BF) isolated from the rhizosphere of a hyperaccumulating plant. The aim is to select microbial consortia with synergistic activity to be used in integrated bioremediation protocols. Pseudomonas fluorescens (Pf), Streptomyces vinaceus (Sv) Penicillium ochrochloron (Po), and Trichoderma harzianum group (Th) were tested in mixes (Po-Sv, Po-Pf, Th-Pf, and Th-Sv). These strains were submitted to tests (agar overlay, agar plug, and distance growth co-growth tests), tailored for this aim, on Czapek yeast agar (CYA) and tryptic soy agar (TSA) media and incubated at 26 - 1 °C for 10 days. BF growth, shape of colonies, area covered on plate, and inhibition capacity were evaluated. Most BF strains still exhibit their typical characters and the colonies separately persisted without inhibition (as Po-Sv) or with reciprocal confinement (as Th-Sv and Th-Pf). Even if apparently inhibited, the Po-Pf mix really merged, thus obtaining morphological traits representing a synergic co-growth, where both strains reached together the maturation phase and developed a sort of mixed biofilm. Indeed, bacterial colonies surround the mature fungal structures adhering to them without any growth inhibition. First data from in vivo experimentation with Po and Pf inocula in pot with metalliferous soils and hyperaccumulator plants showed their beneficial effect on plant growth. However, there is a lack of information regarding the effective co-growth between bacteria and fungi. Indeed, several studies, which directly apply the co-inoculum, do not consider suitable microorganisms consortia. Synergic rhizosphere BFs open new scenarios for plant growth promotion and soil bioremediation. |
| Text | 461378 2021 10.3390/life11040273 Scopus 2 s2.0 85103589218 bacteria fungal interactions biofilmco growth Pseudomonas fluorescens Streptomyces vinaceus Penicillium ochrochloron Trichoderma harzianum grouprhizosphere Frenemies Interactions between rhizospheric bacteria and fungi from metalliferous soils Rosatto S.; Cecchi G.; Roccotiello E.; Piazza S.D.; Cesare A.D.; Mariotti M.G.; Vezzulli L.; Zotti M. Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, Genova, 16132, Laboratory of Plant Biology, DISTAV Department of Earth, Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, 16132 Genova, Italy, , Italy; Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, Genova, 16132, Laboratory of Mycology, DISTAV Department of Earth, Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, 16132 Genova, Italy, , Italy; National Research Council of Italy CNR IRSA , Water Research Institute, Largo Tonolli 50, Verbania, 28922, MEG Molecular Ecology Group, National Research Council of Italy CNR IRSA ,Water Research Institute, Largo Tonolli 50, 28922 Verbania, Italy, , Italy; Laboratory of Microbiology, DISTAV Department of Earth, Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, Genova, 16132, Laboratory of Microbiology, DISTAV Department of Earth, Environment and Life Sciences DISTAV , University of Genoa, Corso Europa 26, 16132 Genova, Italy, , Italy Is it possible to improve the efficiency of bioremediation technologies The use of mixed cultures of bacteria and fungi inoculated at the rhizosphere level could promote the growth of the associated hyperaccumulating plant species and increase the absorption of metals in polluted soils, broadening new horizons on bioremediation purposes. This work investigates interactions between Ni tolerant plant growth promoting bacteria and fungi BF isolated from the rhizosphere of a hyperaccumulating plant. The aim is to select microbial consortia with synergistic activity to be used in integrated bioremediation protocols. Pseudomonas fluorescens Pf , Streptomyces vinaceus Sv Penicillium ochrochloron Po , and Trichoderma harzianum group Th were tested in mixes Po Sv, Po Pf, Th Pf, and Th Sv . These strains were submitted to tests agar overlay, agar plug, and distance growth co growth tests , tailored for this aim, on Czapek yeast agar CYA and tryptic soy agar TSA media and incubated at 26 1 °C for 10 days. BF growth, shape of colonies, area covered on plate, and inhibition capacity were evaluated. Most BF strains still exhibit their typical characters and the colonies separately persisted without inhibition as Po Sv or with reciprocal confinement as Th Sv and Th Pf . Even if apparently inhibited, the Po Pf mix really merged, thus obtaining morphological traits representing a synergic co growth, where both strains reached together the maturation phase and developed a sort of mixed biofilm. Indeed, bacterial colonies surround the mature fungal structures adhering to them without any growth inhibition. First data from in vivo experimentation with Po and Pf inocula in pot with metalliferous soils and hyperaccumulator plants showed their beneficial effect on plant growth. However, there is a lack of information regarding the effective co growth between bacteria and fungi. Indeed, several studies, which directly apply the co inoculum, do not consider suitable microorganisms consortia. Synergic rhizosphere BFs open new scenarios for plant growth promotion and soil bioremediation. 11 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85103589218 origin=inward Articolo in rivista Molecular Diversity Preservation International 2075 1729 Life Basel Life Basel Life Basel Life. Basel andrea.dicesare DI CESARE ANDREA |
