Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples |
| Keywords | CARD-FISHFluorescence signal intensityOligonucleotide probes |
| Year | 2021 |
| Type | Contributo in volume |
| DOI | 10.1007/978-1-0716-1115-9_9 |
| Authors | Matturro, Bruna; Rossetti, Simona; Leitão, Patrícia |
| Text | 461173 2021 10.1007/978 1 0716 1115 9_9 Scopus 2 s2.0 85101475264 CARD FISH Fluorescence signal intensity Oligonucleotide probes CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization CARD FISH for Complex Environmental Samples Matturro, Bruna; Rossetti, Simona; Leitão, Patricia Istituto di Ricerca sulle Acque, Italy; Universidade do Porto CARD FISH technique allows us to increase microbial cell detection compared to traditional FISH assays. Specific nonfluorescent oligonucleotide probes targeting 16S rRNA genes are employed and are chemically activated by the binding of tyramide molecules, with the latter able to generate a cascade of fluorescence signals, improving sensitivity and reducing background noise. The technique has been successfully applied for the detection of microorganisms in different environmental matrices and under different growth conditions including those where cells are characterized by low physiological activity and low ribosome content . This chapter presents a straightforward procedure to execute CARD FISH analysis, from sample preparation and fixation, to microscopic visualization, along with relevant technical notes. Published version http //www.scopus.com/record/display.url eid=2 s2.0 85101475264 origin=inward Capitolo Contributo in volume simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |