| Title | Simultaneous removal of hydrocarbons and sulfate from groundwater using a "bioelectric well" |
| Abstract | Petroleum hydrocarbons (PHs) are often found in groundwater due to human activities like accidental spills, causing health and environmental risks, and requiring remediation. Microbial Electrochemical Technologies (METs) have emerged as a promising alternative to conventional bioremediation techniques for the treatment of PH-contaminated groundwater. However, the field-application of these promising sustainable as well as cost-effective technologies is still scarce. One major reason is the lack of scalable reactor configurations. Herein, an upgraded version of the "bioelectric well", a novel tubular bioelectrochemical reactor that can be installed directly within a groundwater well, was tested for the simultaneous removal of oxidableoxidizable (i.e., toluene and other PH) and reducible (i.e., sulfate) compounds from a real contaminated groundwater. After a proof-of-concept study in batch mode, the system was operated in continuous-flow mode for 48 days with the anode polarized at 0.2 V vs. SHE and a hydraulic retention time of 11 h. In these conditions, a steady-state removal rate of toluene as high as 31 ± 2 mg L d was achieved, which was more than double the value observed with the open circuit potential (OCP) control and one of the highest reported in literature. The electrode polarization went along with a higher abundance of key-functional genes involved in toluene degradation. This was not only showing its clear functional connection to the microbial metabolism, but further allowed to identify the involved electrogenic biodegradation pathway. In addition, the system simultaneously removed sulfate (30 ± 1 mg L d), with bacteria likely using the H generated at the cathode as electron donor. Nevertheless, the apparent sulfate removal rate in the polarized and in the OCP runs was similar. The analysis of the microbial communities evidenced a high abundance of the genus Chlorobium in the effluent of the polarized run. These microorganisms were probably responsible for the continuous oxidative regeneration of sulfate from the sulfide produced at the cathode by sulfate-reducing bacteria. This phenomenon probably hindered the overall removal of sulfate by the bioelectrochemical system. |
| Source | Electrochimica acta 388 |
| Keywords | BioremediationGroundwater remediationMicrobial electrochemical technologiesPetroleum hydrocarbonsToluene |
| Journal | Electrochimica acta |
| Editor | Pergamon Press,, Oxford, Regno Unito |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.electacta.2021.138636 |
| Authors | Tucci, Matteo; Carolina, Cruz Viggi; Resitano, Marco; Matturro, Bruna; Crognale, Simona; Pietrini, Ilaria; Rossetti, Simona; Harnisch, Falk; Aulenta, Federico |
| Text | 461172 2021 10.1016/j.electacta.2021.138636 Scopus 2 s2.0 85108738864 Bioremediation Groundwater remediation Microbial electrochemical technologies Petroleum hydrocarbons Toluene Simultaneous removal of hydrocarbons and sulfate from groundwater using a bioelectric well Tucci, Matteo; Carolina, Cruz Viggi; Resitano, Marco; Matturro, Bruna; Crognale, Simona; Pietrini, Ilaria; Rossetti, Simona; Harnisch, Falk; Aulenta, Federico Istituto di Ricerca sulle Acque, Italy; Helmholtz Zentrum fur Umweltforschung; Eni S.p.A. Decarbonization amp; Environmental R amp;D Petroleum hydrocarbons PHs are often found in groundwater due to human activities like accidental spills, causing health and environmental risks, and requiring remediation. Microbial Electrochemical Technologies METs have emerged as a promising alternative to conventional bioremediation techniques for the treatment of PH contaminated groundwater. However, the field application of these promising sustainable as well as cost effective technologies is still scarce. One major reason is the lack of scalable reactor configurations. Herein, an upgraded version of the bioelectric well , a novel tubular bioelectrochemical reactor that can be installed directly within a groundwater well, was tested for the simultaneous removal of oxidableoxidizable i.e., toluene and other PH and reducible i.e., sulfate compounds from a real contaminated groundwater. After a proof of concept study in batch mode, the system was operated in continuous flow mode for 48 days with the anode polarized at 0.2 V vs. SHE and a hydraulic retention time of 11 h. In these conditions, a steady state removal rate of toluene as high as 31 ± 2 mg L d was achieved, which was more than double the value observed with the open circuit potential OCP control and one of the highest reported in literature. The electrode polarization went along with a higher abundance of key functional genes involved in toluene degradation. This was not only showing its clear functional connection to the microbial metabolism, but further allowed to identify the involved electrogenic biodegradation pathway. In addition, the system simultaneously removed sulfate 30 ± 1 mg L d , with bacteria likely using the H generated at the cathode as electron donor. Nevertheless, the apparent sulfate removal rate in the polarized and in the OCP runs was similar. The analysis of the microbial communities evidenced a high abundance of the genus Chlorobium in the effluent of the polarized run. These microorganisms were probably responsible for the continuous oxidative regeneration of sulfate from the sulfide produced at the cathode by sulfate reducing bacteria. This phenomenon probably hindered the overall removal of sulfate by the bioelectrochemical system. 388 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85108738864 origin=inward Articolo in rivista Pergamon Press, 0013 4686 Electrochimica acta Electrochimica acta Electrochim. acta Electrochimica acta. TUCCI MATTEO simona.rossetti ROSSETTI SIMONA federico.aulenta AULENTA FEDERICO bruna.matturro MATTURRO BRUNA carolina.cruzviggi CRUZ VIGGI CAROLINA simona.crognale CROGNALE SIMONA |
