Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD-FISH and real time PCR |
| Abstract | Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in contaminant degradation processes. However, despite the fact that significant efforts have been taken to make these tools more reliable and specific, their application for the analysis of field samples is hardly ever applied. In this study, a combination of three methods (CARD-FISH, qPCR and RT-qPCR) was successfully applied to evaluate the distribution and the activity of known chlorinated solvent dechlorinating bacteria in a contaminated site where no remedial actions have been undertaken. CAtalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) specifically provided the cell densities of known dechlorinating bacteria and was found to be more sensitive than quantitative PCR (qPCR) for the quantification of 'Dehalococcoides' cell numbers in the aquifer. Among the screened dechlorinators, 'Dehalococcoides' spp. were mainly found and nearly homogenously distributed in the aquifers at concentrations ranging from 8.1×10(5)±1.2×10(5) to 2.5×10(7)±5.6×10(6)cells per liter of groundwater (with a relative abundance out of the total Bacteria of 0.7-15%). Further, the dechlorination potentialities of 'Dehalococcoides' species living in the aquifer were evaluated by analyzing the abundance and the expression of 16S rRNA genes and reductive dehalogenase (RDase) encoding functional genes by qPCR and Reverse Transcription qPCR (RT-qPCR). 'Dehalococcoides'tceA gene, known to be associated to strains capable of reducing chlorinated solvents beyond cis-DCE, was found and expressed in the field. Overall, this study proved the existence of a well-established dechlorinating microbial community able to use contaminants as substrates for their metabolic activity and indicated the occurrence of reductive dechlorination at the site. |
| Source | New biotechnology (Online) |
| Keywords | Dehalococcoidesbioremediationbiomolecular tools |
| Journal | New biotechnology (Online) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2012 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2012.07.006 |
| Authors | B Matturro, F Aulenta, M Majone, M Petrangeli Papini, V Tandoi, S Rossetti |
| Text | 461171 2012 10.1016/j.nbt.2012.07.006 Scopus 2 s2.0 84867654413 PubMed 26748009 Dehalococcoides bioremediation biomolecular tools Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD FISH and real time PCR B Matturro, F Aulenta, M Majone, M Petrangeli Papini, V Tandoi, S Rossetti Water Research Institute, CNR, Via Salaria km 29.300, Monterotondo RM 00015, Italy. Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in contaminant degradation processes. However, despite the fact that significant efforts have been taken to make these tools more reliable and specific, their application for the analysis of field samples is hardly ever applied. In this study, a combination of three methods CARD FISH, qPCR and RT qPCR was successfully applied to evaluate the distribution and the activity of known chlorinated solvent dechlorinating bacteria in a contaminated site where no remedial actions have been undertaken. CAtalysed Reporter Deposition Fluorescence In Situ Hybridization CARD FISH specifically provided the cell densities of known dechlorinating bacteria and was found to be more sensitive than quantitative PCR qPCR for the quantification of Dehalococcoides cell numbers in the aquifer. Among the screened dechlorinators, Dehalococcoides spp. were mainly found and nearly homogenously distributed in the aquifers at concentrations ranging from 8.1×10 5 ±1.2×10 5 to 2.5×10 7 ±5.6×10 6 cells per liter of groundwater with a relative abundance out of the total Bacteria of 0.7 15% . Further, the dechlorination potentialities of Dehalococcoides species living in the aquifer were evaluated by analyzing the abundance and the expression of 16S rRNA genes and reductive dehalogenase RDase encoding functional genes by qPCR and Reverse Transcription qPCR RT qPCR . Dehalococcoides tceA gene, known to be associated to strains capable of reducing chlorinated solvents beyond cis DCE, was found and expressed in the field. Overall, this study proved the existence of a well established dechlorinating microbial community able to use contaminants as substrates for their metabolic activity and indicated the occurrence of reductive dechlorination at the site. Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84867654413 partnerID=MN8TOARS Articolo in rivista Elsevier 1876 4347 New biotechnology Online New biotechnology Online New biotechnology. Online simona.rossetti ROSSETTI SIMONA federico.aulenta AULENTA FEDERICO bruna.matturro MATTURRO BRUNA |