| Title | Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications |
| Abstract | The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view. |
| Source | Water (Basel) |
| Keywords | polyhydroxyalkanoatesmixed microbial culturesacidogenic fermentationelectron-donorsbioremediation |
| Journal | Water (Basel) |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.3390/w13070897 |
| Authors | Neda Amanat, Bruna Matturro, Marta Maria Rossi, Francesco Valentino, Marianna Villano, Marco Petrangeli Papini |
| Text | 461170 2021 10.3390/w13070897 polyhydroxyalkanoates mixed microbial cultures acidogenic fermentation electron donors bioremediation Assessment of Long Term Fermentability of PHA Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications Neda Amanat, Bruna Matturro, Marta Maria Rossi, Francesco Valentino, Marianna Villano, Marco Petrangeli Papini Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Water Research Institute IRSA CNR , Via Salaria km 29.300, 00015 Monterotondo RM, Italy. Department of Environmental Sciences, Informatics and Statistics, Ca Foscari University of Venice, Via Torino 155, 30172 Mestre Venice, Italy. The use of polyhydroxyalkanoates PHA as slow release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA based materials has been investigated in batch and continuous flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures MMC using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids VFA production was observed with a low purity MMC deriving material, consisting of microbial cells containing 56% on weight basis of intracellular PHA. Indeed, with this material a sustained, long term production of organic acids i.e., acetic, propionic, and butyric acids was observed. In addition, the obtained yield of conversion into acids up to 70% gVFA/gPHA was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view. Published version https //doi.org/10.3390/w13070897 Articolo in rivista Molecular Diversity Preservation International 2073 4441 Water Basel Water Basel Water Basel Water. Basel bruna.matturro MATTURRO BRUNA |
