Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Correlations between maximum reductive dechlorination rates and specific biomass parameters in Dehalococcoides mccartyi consortia enriched on chloroethenes PCE, TCE and cis-1,2-DCE |
| Abstract | One of the challenges to implementing the modeling of the biological reductive dechlorination (RD) process is the evaluation of biological parameters that represent the abundance/activity levels of the microorganisms involved in the biodegradation of chloroethenes. Here we report a combined analysis of kinetic and specific biomass parameters conducted on three dechlorinating consortia enriched on PCE, TCE and cis-1,2-DCE. In these consortia, Dehalococcoides mccartyi (Dhc) represented >=70% of the bacterial population identified via 16S rRNA gene amplicon sequencing. Quantitative biomolecular methods were used to generate specific biomass parameters targeting either the Dhc population (16S rRNA genes or cells) or specific genes encoding RD process-involved reductive dehalogenases. The correlation factor between the abundance of active Dhc cells or tceA gene copies and maximum RD rates allowed to predict an increment of 7E+09 of active Dhc cells or 5E+09 tceA gene copies/L under controlled conditions. Diversely, the utilization of gene transcripts as biomass parameters for RD modeling did not provide reliable correlations with kinetic performances. This study provides valuable insights for further modeling of the RD process through the utilization of specific biomass parameters. |
| Source | FEMS microbiology, ecology (print) |
| Keywords | Dehalococcoides mccartyibioremediationspecific biomass parameterschlorinated ethenesreductive dechlorination |
| Journal | FEMS microbiology, ecology (print) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.1093/femsec/fiab064 |
| Authors | Matturro Bruna, Majone Mauro, Aulenta Federico, Rossetti Simona |
| Text | 461169 2021 10.1093/femsec/fiab064 Dehalococcoides mccartyi bioremediation specific biomass parameters chlorinated ethenes reductive dechlorination Correlations between maximum reductive dechlorination rates and specific biomass parameters in Dehalococcoides mccartyi consortia enriched on chloroethenes PCE, TCE and cis 1,2 DCE Matturro Bruna, Majone Mauro, Aulenta Federico, Rossetti Simona Water Research Institute, IRSA CNR, Via Salaria km 29.300, Monterotondo RM 00015, Italy. Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. One of the challenges to implementing the modeling of the biological reductive dechlorination RD process is the evaluation of biological parameters that represent the abundance/activity levels of the microorganisms involved in the biodegradation of chloroethenes. Here we report a combined analysis of kinetic and specific biomass parameters conducted on three dechlorinating consortia enriched on PCE, TCE and cis 1,2 DCE. In these consortia, Dehalococcoides mccartyi Dhc represented >=70% of the bacterial population identified via 16S rRNA gene amplicon sequencing. Quantitative biomolecular methods were used to generate specific biomass parameters targeting either the Dhc population 16S rRNA genes or cells or specific genes encoding RD process involved reductive dehalogenases. The correlation factor between the abundance of active Dhc cells or tceA gene copies and maximum RD rates allowed to predict an increment of 7E 09 of active Dhc cells or 5E 09 tceA gene copies/L under controlled conditions. Diversely, the utilization of gene transcripts as biomass parameters for RD modeling did not provide reliable correlations with kinetic performances. This study provides valuable insights for further modeling of the RD process through the utilization of specific biomass parameters. Published version https //doi.org/10.1093/femsec/fiab064 Articolo in rivista Elsevier 0168 6496 FEMS microbiology, ecology print FEMS microbiology, ecology print FEMS microbiol. ecol. print FEMS microbiology, ecology. print Federation of European Microbiological Societies microbiology, ecology print simona.rossetti ROSSETTI SIMONA federico.aulenta AULENTA FEDERICO bruna.matturro MATTURRO BRUNA |