| Title | A novel extraction process of phycobiliproteins with high purity grade from biomass of Arthrospira platensis and Porphyridium cruentum |
| Abstract | Phycobiliproteins are water soluble proteins, which are brilliantly colored, highly fluorescent
pigments of the photosynthetic light-harvesting antenna complexes of cyanobacteria and some
algae, having many commercial applications in biomedicine, pharmaceutics, cosmetics and
foods.
Currently, a simple, reliable, not time consuming, not expensive method to achieve a large-scale
sustainable production of high-value phycobiliproteins is a matter of great concern for industry.
Here we present a new extraction process [1] developed to extract, with high yield,
phycobiliproteins from Arthrospira platensis (Spirulina) and Porphyridium cruentum biomass.
The major element of originality and innovation of the process, carried out entirely in aqueous
solution, is the exploitation of a new approach in which 1) the traditional sequence of extraction
followed by purification is inverted, 2) cell rupture and purification of the product are merged in a
single step.
Cell rupture and purification of the product were carried out by ultrasonication in an ammonium
sulphate solution, upstream of the pigment extraction process. The phycobiliproteins were
extracted/recovered subsequently, obtaining a highly concentrated extract (a few mg mL ) and
relatively high purity (P >= 2, usually 2 < P < 4) without downstream purification. Production times
were reduced, to the advantage of the product quality. In fact, the reduced process time avoided
the development of the unpleasant smell that often characterizes crude extracts obtained by
applying long lasting extraction procedures, particularly at room temperature.
The process has been applied to both fresh and dry biomass, obtaining better results with fresh
biomass. |
| Source | International Conference on Algal Biomass, Biofuels and Bioproducts, virtual, 14-16 giugno 2021 |
| Keywords | Phycobiliproteinsextractioninnovative process |
| Year | 2021 |
| Type | Presentazione |
| Authors | Rosaria Lauceri, Graziella Chini Zittelli, Giuseppe Torzillo |
| Text | 461047 2021 Phycobiliproteins extraction innovative process A novel extraction process of phycobiliproteins with high purity grade from biomass of Arthrospira platensis and Porphyridium cruentum Rosaria Lauceri, Graziella Chini Zittelli, Giuseppe Torzillo CNR IRSA, CNR IBE Phycobiliproteins are water soluble proteins, which are brilliantly colored, highly fluorescent pigments of the photosynthetic light harvesting antenna complexes of cyanobacteria and some algae, having many commercial applications in biomedicine, pharmaceutics, cosmetics and foods. Currently, a simple, reliable, not time consuming, not expensive method to achieve a large scale sustainable production of high value phycobiliproteins is a matter of great concern for industry. Here we present a new extraction process 1 developed to extract, with high yield, phycobiliproteins from Arthrospira platensis Spirulina and Porphyridium cruentum biomass. The major element of originality and innovation of the process, carried out entirely in aqueous solution, is the exploitation of a new approach in which 1 the traditional sequence of extraction followed by purification is inverted, 2 cell rupture and purification of the product are merged in a single step. Cell rupture and purification of the product were carried out by ultrasonication in an ammonium sulphate solution, upstream of the pigment extraction process. The phycobiliproteins were extracted/recovered subsequently, obtaining a highly concentrated extract a few mg mL and relatively high purity P >= 2, usually 2 < P < 4 without downstream purification. Production times were reduced, to the advantage of the product quality. In fact, the reduced process time avoided the development of the unpleasant smell that often characterizes crude extracts obtained by applying long lasting extraction procedures, particularly at room temperature. The process has been applied to both fresh and dry biomass, obtaining better results with fresh biomass. Preprint International Conference on Algal Biomass, Biofuels and Bioproducts virtual 14 16 giugno 2021 Internazionale Contributo Lauceri AlgalBBB2021 Session 8A presentazione avente come oggetto un metodo innovativo per estrarre ficobiliproteine in resa elevata ottenendo prodotti caratterizzati da un elevato grado di purezza a da un elevata concentrazione dei pigmenti. Lauceri AlgalBBB2021 Session 8A.pdf Presentazione rosaria.lauceri LAUCERI ROSARIA graziella.chinizittelli CHINI ZITTELLI GRAZIELLA giuseppe.torzillo TORZILLO GIUSEPPE |
