Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbial Community Successional Changes in a Full-Scale Mesophilic Anaerobic Digester from the Start-Up to the Steady-State Conditions |
| Abstract | Anaerobic digestion is a widely used technology for sewage sludge stabilization and biogas production. Although the structure and composition of the microbial communities responsible for the process in full-scale anaerobic digesters have been investigated, little is known about the microbial successional dynamics during the start-up phase and the response to variations occurring in such systems under real operating conditions. In this study, bacterial and archaeal population dynamics of a full-scale mesophilic digester treating activated sludge were investigated for the first time from the start-up, performed without adding external inoculum, to steady-state operation. High-throughput 16S rRNA gene sequencing was used to describe the microbiome evolution. The large majority of the reads were affiliated to fermentative bacteria. Bacteroidetes increased over time, reaching 22% of the total sequences. Furthermore, Methanosaeta represented the most abundant methanogenic component. The specific quantitative data generated by real-time PCR indicated an enrichment of bacteria and methanogens once the steady state was reached. The analysis allowed evaluation of the microbial components more susceptible to the shift from aerobic to anaerobic conditions and estimation of the microbial components growing or declining in the system. Additionally, activated sludge was investigated to evaluate the microbial core selected by the WWTP operative conditions. |
| Source | Microorganisms 9 |
| Keywords | waste-activated sludgefull-scale plantanaerobic digestion16S rRNA gene sequencingmicrobial population dynamics |
| Journal | Microorganisms |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.3390/ microorganisms9122581 |
| Authors | Barbara Tonanzi, Simona Crognale, Andrea Gianico, Stefano Della Sala, Paola Miana, Maria Chiara Zaccone and Simona Rossetti |
| Text | 460729 2021 10.3390/ microorganisms9122581 waste activated sludge full scale plant anaerobic digestion 16S rRNA gene sequencing microbial population dynamics Microbial Community Successional Changes in a Full Scale Mesophilic Anaerobic Digester from the Start Up to the Steady State Conditions Barbara Tonanzi, Simona Crognale, Andrea Gianico, Stefano Della Sala, Paola Miana, Maria Chiara Zaccone and Simona Rossetti National Research Council of Italy Water Research Institute CNR IRSA, Area della Ricerca RM1, 00015 Monterotondo, Rome, Italy. Veritas S.p.a., 30135 Venezia, Italy. Anaerobic digestion is a widely used technology for sewage sludge stabilization and biogas production. Although the structure and composition of the microbial communities responsible for the process in full scale anaerobic digesters have been investigated, little is known about the microbial successional dynamics during the start up phase and the response to variations occurring in such systems under real operating conditions. In this study, bacterial and archaeal population dynamics of a full scale mesophilic digester treating activated sludge were investigated for the first time from the start up, performed without adding external inoculum, to steady state operation. High throughput 16S rRNA gene sequencing was used to describe the microbiome evolution. The large majority of the reads were affiliated to fermentative bacteria. Bacteroidetes increased over time, reaching 22% of the total sequences. Furthermore, Methanosaeta represented the most abundant methanogenic component. The specific quantitative data generated by real time PCR indicated an enrichment of bacteria and methanogens once the steady state was reached. The analysis allowed evaluation of the microbial components more susceptible to the shift from aerobic to anaerobic conditions and estimation of the microbial components growing or declining in the system. Additionally, activated sludge was investigated to evaluate the microbial core selected by the WWTP operative conditions. 9 Published version https //www.mdpi.com/2076 2607/9/12/2581 Microbial Community Successional Changes in a Full Scale Mesophilic Anaerobic Digester from the Start Up to the Steady State Conditions Manuscript 2021 Tonanzi et al VERITAS microorganisms.pdf Articolo in rivista Molecular Diversity Preservation International 2076 2607 Microorganisms Microorganisms barbara.tonanzi TONANZI BARBARA simona.rossetti ROSSETTI SIMONA andrea.gianico GIANICO ANDREA simona.crognale CROGNALE SIMONA |