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DatoValore
TitleDeliverable D2.13 Report on physical, chemical and microbiological characteristics of the residues (biomass)
KeywordsIn Situ Hybridization TechniquesMBRBIOZO-DETOXPharmaceutical WastewaterLandfill Leachate
Year2011
TypeRapporto di progetto (Project report)
AuthorsC.M. Braguglia, M. De Sanctis, A. Gianico , G. Mininni, S. Rossetti
Text458510 2011 In Situ Hybridization Techniques MBR BIOZO DETOX Pharmaceutical Wastewater Landfill Leachate Deliverable D2.13 Report on physical, chemical and microbiological characteristics of the residues biomass C.M. Braguglia, M. De Sanctis, A. Gianico , G. Mininni, S. Rossetti IRSA CNR In situ hybridization techniques and conventional/epifluorescence microscopic analysis have been applied for the biomolecular characterization of mixed biomass occurring in the biological units of the technologies investigated within WP2. In particular, MBR IRSA CNR, Bari and BIOZO DETOX NIVA systems were monitored by FISH Fluorescence In Situ Hybridization for determining the abundance and the composition of the microbial communities degrading pharmaceutical and landfill leachate wastewaters respectively. The analysed samples from MBR reactor treating pharmaceutical wastewater showed the occurrence of highly selected mixed cultures mainly composed by a filamentous bacterium known as Nostocoida limicola belonging to Alphaproteobacteria , previously found as dominant filament causing bulking problems in industrial wastewater systems due to its capability to store high amount of carbon as polyhydroxyalkanoates under aerobic conditions. A negligible effect on biomass composition was further observed after the adoption of the AOP processes. Besides the biomolecular characterization, a set of chemical physical analyses were carried out on MBR sludge samples, before and after the integration of the advanced oxidation process UV H2O2 . The selected parameters were Total and Volatile Solids, soluble COD, Dissolved Oxygen, Capillary Suction Time and pH. Moreover the Heavy Metals class of pollutants inserted on the 3rd Draft Working Document on Sludge, 2000 concentration of the MBR sludge samples was measured to assess the sludge quality for agricultural use. The biomolecular characterization of the biofilm growing in BIOZO DETOX systems was mainly focused on the estimation of the nitrifying and denitrifying microbial populations. Some differences in terms of microbial activity were also observed among reactors six in series reactors, three anoxic and three aerated respectively due to the differently applied contaminant loading conditions and carbon substrate availability. Rapporto finale di progetto Deliverable D2.13 Project Deliverable D2.13 INNOVATECH.pdf Rapporto di progetto Project report giuseppe.mininni MININNI GIUSEPPE simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA marco.desanctis DE SANCTIS MARCO INNOWATECH Innovative and integrated technologies for the treatment of industrial wastewater