| Title | Expression of genes involved in oxidative stress response in colonies of the ascidian Botryllus schlosseri exposed to various environmental conditions |
| Abstract | Environmental stress conditions are ultimately related to the induction of oxidative stress in organisms, as a consequence of an increased production of reactive oxygen species (ROS). This could be exploited to study sub-lethal effects induced by the environment in the organisms. In the present work, we evaluate the possibility to use the colonial ascidian Botryllus schlosseri as a bioindicator, to assess the environmental quality in the Lagoon of Venice. Three colony batches were immersed, for 22 days, at two sites (1 and 2) with different grades of hydrodynamics and anthropogenic impact and physico-chemical features of seawater; a control batch was kept in a large tank with continuous seawater flow at the Marine Station of the Department of Biology, University of Padova, in Chioggia (site 3). Seawater at site 2 had higher pH and temperature than site 1. Colonies were then retrieved, their mRNA was extracted and the level of transcription of genes involved in oxidative stress response (glutathione synthase, ?-glutamyl-cysteine ligase, modulatory subunit, two isoforms of glutathione peroxidases and Cu/Zn superoxide dismutase) was evaluated. In colonies from sites 1 and 2, most genes showed significantly increased transcriptional levels with respect to control values. Spectrophotometric analyses of colony homogenates revealed that the enzymatic activity of superoxide dismutase and catalase was higher in colonies from site 2 as compared to site 1, allowing us to speculate that colonies in site 2 were under higher stress level than those in site 1. Overall, we can conclude that B. schlosseri seems a good indicator of the ecological status of the Lagoon environment, within a range of pH and temperature in which colonies are used to live. |
| Source | Estuarine, coastal and shelf science (Print) 187, pp. 22–27 |
| Keywords | Botryllus schlosseriOxidative stressBiomarkersGSHGPxSOD |
| Journal | Estuarine, coastal and shelf science (Print) |
| Editor | Academic Press in association with the Estuarine and Brackish-water Sciences Association,, New York, Stati Uniti d'America |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.ecss.2016.12.017 |
| Authors | Tasselli S.; Ballin F.; Franchi N.; Fabbri E.; Ballarin L. |
| Text | 453439 2017 10.1016/j.ecss.2016.12.017 Scopus 2 s2.0 85009060593 Botryllus schlosseri Oxidative stress Biomarkers GSH GPx SOD Expression of genes involved in oxidative stress response in colonies of the ascidian Botryllus schlosseri exposed to various environmental conditions Tasselli S.; Ballin F.; Franchi N.; Fabbri E.; Ballarin L. University of Padova, Department of Biology, Via Ugo Bassi 58/B, Padova, 35131, , Italy; University of Bologna, Department of Biological, Geological and Environmental Science, Via Selmi 3, Bologna, , Italy Environmental stress conditions are ultimately related to the induction of oxidative stress in organisms, as a consequence of an increased production of reactive oxygen species ROS . This could be exploited to study sub lethal effects induced by the environment in the organisms. In the present work, we evaluate the possibility to use the colonial ascidian Botryllus schlosseri as a bioindicator, to assess the environmental quality in the Lagoon of Venice. Three colony batches were immersed, for 22 days, at two sites 1 and 2 with different grades of hydrodynamics and anthropogenic impact and physico chemical features of seawater; a control batch was kept in a large tank with continuous seawater flow at the Marine Station of the Department of Biology, University of Padova, in Chioggia site 3 . Seawater at site 2 had higher pH and temperature than site 1. Colonies were then retrieved, their mRNA was extracted and the level of transcription of genes involved in oxidative stress response glutathione synthase, glutamyl cysteine ligase, modulatory subunit, two isoforms of glutathione peroxidases and Cu/Zn superoxide dismutase was evaluated. In colonies from sites 1 and 2, most genes showed significantly increased transcriptional levels with respect to control values. Spectrophotometric analyses of colony homogenates revealed that the enzymatic activity of superoxide dismutase and catalase was higher in colonies from site 2 as compared to site 1, allowing us to speculate that colonies in site 2 were under higher stress level than those in site 1. Overall, we can conclude that B. schlosseri seems a good indicator of the ecological status of the Lagoon environment, within a range of pH and temperature in which colonies are used to live. 187 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85009060593 origin=inward Articolo in rivista Academic Press in association with the Estuarine and Brackish water Sciences Association, 0272 7714 Estuarine, coastal and shelf science Print Estuarine, coastal and shelf science Print Estuar., coast. shelf sci. Print Estuarine, coastal and shelf science. Print stefanotasselli TASSELLI STEFANO |
