| Title | The interactions of fullerene C60 and Benzo(?)pyrene influence their bioavailability and toxicity to zebrafish embryos |
| Abstract | This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles (C) and Benzo(?)pyrene (B(?)P) on zebrafish embryos, which were exposed to C and B(?)P alone and to C doped with B(?)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C interaction with B(?)P. The carrier role of C for B(?)P was observed, however adsorption on C did not affect the accumulation and localization of B(?)P in the embryos. Instead, C doped with B(?)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C alone. As for toxicity, our results suggested that C alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C + B(?)P induced cellular response mechanisms similar to B(?)P alone, but generating greater cellular damages in the exposed embryos. Once C nanoparticles and B(?)P meet in water, they reciprocally affect their bioavailability and, by consequence, their toxicity to organisms. |
| Source | Environmental pollution (1987) 241, pp. 999–1008 |
| Keywords | Fullerene nanoparticlesDanio rerioOxidative stressProteomicsTrojan horse effect |
| Journal | Environmental pollution (1987) |
| Editor | Elsevier Applied Science Publishers, Barking, Regno Unito |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.envpol.2018.06.042 |
| Authors | Della Torre C.; Maggioni D.; Ghilardi A.; Parolini M.; Santo N.; Landi C.; Madaschi L.; Magni S.; Tasselli S.; Ascagni M.; Bini L.; La Porta C.; Del Giacco L.; Binelli A. |
| Text | 453438 2018 10.1016/j.envpol.2018.06.042 Scopus 2 s2.0 85048721111 Fullerene nanoparticles Danio rerio Oxidative stress Proteomics Trojan horse effect The interactions of fullerene C60 and Benzo pyrene influence their bioavailability and toxicity to zebrafish embryos Della Torre C.; Maggioni D.; Ghilardi A.; Parolini M.; Santo N.; Landi C.; Madaschi L.; Magni S.; Tasselli S.; Ascagni M.; Bini L.; La Porta C.; Del Giacco L.; Binelli A. Department of Biosciences, University of Milan, , Italy; Department of Chemistry, University of Milan, , Italy; Department of Environmental Science and Policy, University of Milan, , Italy; Department of Life Science, University of Siena, , Italy; CNR IRSA National Research Council Water Research Institute , Brugherio, , Italy This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles C and Benzo pyrene B P on zebrafish embryos, which were exposed to C and B P alone and to C doped with B P. The uptake of pollutants into their tissues and intra cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C interaction with B P. The carrier role of C for B P was observed, however adsorption on C did not affect the accumulation and localization of B P in the embryos. Instead, C doped with B P resulted more prone to sedimentation and less bioavailable for the embryos compared to C alone. As for toxicity, our results suggested that C alone elicited oxidative stress in embryos and a down regulation of proteins involved in energetic metabolism. The C B P induced cellular response mechanisms similar to B P alone, but generating greater cellular damages in the exposed embryos. Once C nanoparticles and B P meet in water, they reciprocally affect their bioavailability and, by consequence, their toxicity to organisms. 241 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85048721111 origin=inward Articolo in rivista Elsevier Applied Science Publishers 0269 7491 Environmental pollution 1987 Environmental pollution 1987 Environ. pollut. 1987 Environmental pollution 1987 stefanotasselli TASSELLI STEFANO |
