Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Direct conversion of food waste extract into caproate: Metagenomics assessment of chain elongation process |
| Abstract | In a circular economy strategy, waste resources can be used for the biological production of high added-value substances, such as medium chain fatty acids (MCFAs), thus minimising waste and favouring a sustainable process. This study investigates single-stage fermentation processes for the production of MCFAs in a semi-continuous reactor treating the extract of real food waste (FW), without the addition of external electron donors. Two sequential acidogenic fermentation tests were carried out at an organic loading rate (OLR) of 5 and 15 gCOD Ld with a hydraulic retention time of 4 days and pH controlled at 6 ± 0.2. The highest level of caproate (4.8 g L ) was observed at OLR of 15 gCOD Ld with a microbiome mainly composed by lactate-producing Actinomyces, Atopobium, and Olsenella species and caproate-producing Pseudoramibacter. Metagenomic analysis revealed the presence of key enzymes for the production of lactate, such as lactate dehydrogenase and pyruvate ferredoxin oxidoreductase, as well as several enzymes involved in the reverse ?-oxidation pathway, thus suggesting the occurrence of a lactate-based chain elongation process. |
| Source | Microorganisms 9, pp. 1–20 |
| Keywords | medium chain fatty acidsmicrobial chain elongationcaproatewaste valorisationmetagenomics |
| Journal | Microorganisms |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.3390/microorganisms9020327 |
| Authors | Crognale S.; Braguglia C.M.; Gallipoli A.; Gianico A.; Rossetti S.; Montecchio D. |
| Text | 453161 2021 10.3390/microorganisms9020327 Scopus 2 s2.0 85100490104 ISI Web of Science WOS 000622840100001 medium chain fatty acids microbial chain elongation caproate waste valorisation metagenomics Direct conversion of food waste extract into caproate Metagenomics assessment of chain elongation process Crognale S.; Braguglia C.M.; Gallipoli A.; Gianico A.; Rossetti S.; Montecchio D. Water Research Institute, National Research Council of Italy IRSA CNR , Via Salaria, km 29.300, 00015 Monterotondo, Roma, Italy In a circular economy strategy, waste resources can be used for the biological production of high added value substances, such as medium chain fatty acids MCFAs , thus minimising waste and favouring a sustainable process. This study investigates single stage fermentation processes for the production of MCFAs in a semi continuous reactor treating the extract of real food waste FW , without the addition of external electron donors. Two sequential acidogenic fermentation tests were carried out at an organic loading rate OLR of 5 and 15 gCOD Ld with a hydraulic retention time of 4 days and pH controlled at 6 ± 0.2. The highest level of caproate 4.8 g L was observed at OLR of 15 gCOD Ld with a microbiome mainly composed by lactate producing Actinomyces, Atopobium, and Olsenella species and caproate producing Pseudoramibacter. Metagenomic analysis revealed the presence of key enzymes for the production of lactate, such as lactate dehydrogenase and pyruvate ferredoxin oxidoreductase, as well as several enzymes involved in the reverse oxidation pathway, thus suggesting the occurrence of a lactate based chain elongation process. 9 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85100490104 origin=inward Direct Conversion of Food Waste Extract into Caproate Metagenomics Assessment of Chain Elongation Process Manuscript 2021 Crognale microorganisms 09 00327.pdf Articolo in rivista Molecular Diversity Preservation International 2076 2607 Microorganisms Microorganisms simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA daniele.montecchio MONTECCHIO DANIELE agata.gallipoli GALLIPOLI AGATA simona.crognale CROGNALE SIMONA |