| Authors | Laura Moro, Gianni Pezzotti, Mehmet Turemis, Josep Sanchís, Marinella Farré, Renata Denaro, Maria Grazia Giacobbe, Francesca Crisafi, Maria Teresa Giardi, |
| Text | 450280 2018 10.1016/j.snb.2018.04.111 ISI Web of Science WOS 000434011500051 Optical biosensor Marine monitoring Chemical pollutants Pesticides Microalgae Alga paramecium symbiosis Fast pesticide pre screening in marine environment using a green microalgae based optical bioassay Laura Moro, Gianni Pezzotti, Mehmet Turemis, Josep Sanchis, Marinella Farre, Renata Denaro, Maria Grazia Giacobbe, Francesca Crisafi, Maria Teresa Giardi, Biosensor Srl, Via degli Olmetti 44, 00060 Formello, Rome, Italy Water and Soil Quality Research Group, Institute of Environmental Assessment and Water Research IDAEA CSIC , c/ Jordi Girona 18 26, 08034 Barcelona, Spain Institute for Coastal Marine Environment IAMC CNR , Spianata S. Raineri 86, 98122 Messina, Italy Crystallography Institute, CNR Area della Ricerca di Roma, Via Salaria km 29,300, 00015 Monterotondo, Rome, Italy Unprecedented increases in anthropogenic pollution of marine environments have made the development of sensitive, cheap, and adaptable early warning systems absolutely crucial for on site monitoring of chemical contaminants. Biosensors based on microalgae cells might constitute a promising and cost effective alternative for environmental analyses since they capable of providing rapid toxicity information in the case of pollution, while assessing the harmful effects of the contaminants on the ecosystem. In this work, an optical biosensor, based on an array of algal biomediators, has been developed to monitor relevant marine pollutants. A pre study was performed to select the most suitable biomediator for measurements of pesticides in marine water. The potential of the algae protozoa symbiotic association between Chlorella vulgaris and the ciliate Tetrahymena pyriformis as a sensitive biomediator for biosensor development was assessed, showing enhanced resistance to marine water salinity compared to free living algae strains. The symbiotic strain was entrapped in calcium alginate within newly developed fluidic flow cells with integrated detectors for real time detection of marine pollutants by fluorescence analysis of photosynthetic photosystem II. The biosensor response was examined in the presence of three commonly found pesticides simazine, atrazine and diuron alone or in combination, and the results were compared by liquid chromatography mass spectrometry. The limits of detection for the three herbicides were 1.35, 0.44, and 0.25 mu g/L respectively, and 0.13 mu g/L for the mixture. 270 Published version Fast pesticide pre screening in marine environment using a green microalgae based optical bioassay Articolo pubblicato in pdf Fast pesticide pre screening in marine environment using a green.pdf Articolo in rivista Elsevier Sequoia 0925 4005 Sensors and actuators. B, Chemical Print Sensors and actuators. B, Chemical Print Sens. actuators, B, Chem. Print Sensors and actuators. Print Sensors and actuators B Print gianni.pezzottiescobar PEZZOTTI ESCOBAR GIANNI maria.giacobbe GIACOBBE MARIA renata.denaro DENARO RENATA francesca.crisafi CRISAFI FRANCESCA |
