| Abstract | Cannabis sativa L. (hemp) is a versatile plant which can adapt to various environmental conditions. Hempseeds provide high quality lipids, mainly represented by polyunsaturated acids, and highly digestible proteins rich of essential aminoacids. Hempseed composition can vary according to plant genotype, but other factors such as agronomic and climatic conditions can affect the presence of nutraceutic compounds. In this research, seeds from two cultivars of C. sativa (Futura 75 and Finola) grown in a mountain environment of the Italian Alps were analyzed. The main purpose of this study was to investigate changes in the protein profile of seeds obtained from such environments, using two methods (sequential and total proteins) for protein extraction and two analytical approaches SDS-PAGE and 2D-gel electrophoresis, followed by protein identification by mass spectrometry. The fatty acids profile and carotenoids content were also analysed. Mountain environments mainly affected fatty acid and protein profiles of Finola seeds. These changes were not predictable by the sole comparison of certified seeds from Futura 75 and Finola cultivars. The fatty acid profile confirmed a high PUFA content in both cultivars from mountain area, while protein analysis revealed a decrease in the protein content of Finola seeds from the experimental fields. |
| Text | 449712 2021 10.1038/s41598 021 83290 1 Scopus 2 s2.0 85101178740 Proteins; hempseeds; mountain environment; bioactive compounds Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment Cattaneo C.; Givonetti A.; Leoni V.; Guerrieri N.; Manfredi M.; Giorgi A.; Cavaletto M. Dipartimento di Scienze e Innovazione Tecnologica DiSIT, Universita del Piemonte Orientale, Vercelli, 13100, Dipartimento di Scienze e Innovazione Tecnologica DiSIT, Universita del Piemonte Orientale, 13100, Vercelli, Italy, , Italy; Centre of Applied Studies for the Sustainable Management and Protection of Mountain Areas CRC Ge.S.Di.Mont. , University of Milan, Via Morino 8, Edolo, BS , 25048, Centre of Applied Studies for the Sustainable Management and Protection of Mountain Areas CRC Ge.S.Di.Mont. , University of Milan, Via Morino 8, 25048, Edolo, BS , Italy, , Italy; Water Research Institute National Research Council IRSA CNR , Verbania, Water Research Institute National Research Council IRSA CNR , Verbania, Italy, , Italy; Centro di Ricerca Traslazionale sulle Malattie Autoimmuni e Allergiche CAAD, Universita del Piemonte Orientale, Novara, 28100, Centro di Ricerca Traslazionale sulle Malattie Autoimmuni e Allergiche CAAD, Universita del Piemonte Orientale, 28100, Novara, Italy, , Italy Cannabis sativa L. hemp is a versatile plant which can adapt to various environmental conditions. Hempseeds provide high quality lipids, mainly represented by polyunsaturated acids, and highly digestible proteins rich of essential aminoacids. Hempseed composition can vary according to plant genotype, but other factors such as agronomic and climatic conditions can affect the presence of nutraceutic compounds. In this research, seeds from two cultivars of C. sativa Futura 75 and Finola grown in a mountain environment of the Italian Alps were analyzed. The main purpose of this study was to investigate changes in the protein profile of seeds obtained from such environments, using two methods sequential and total proteins for protein extraction and two analytical approaches SDS PAGE and 2D gel electrophoresis, followed by protein identification by mass spectrometry. The fatty acids profile and carotenoids content were also analysed. Mountain environments mainly affected fatty acid and protein profiles of Finola seeds. These changes were not predictable by the sole comparison of certified seeds from Futura 75 and Finola cultivars. The fatty acid profile confirmed a high PUFA content in both cultivars from mountain area, while protein analysis revealed a decrease in the protein content of Finola seeds from the experimental fields. 11 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85101178740 origin=inward Articolo in rivista Nature Publishing Group 2045 2322 Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group nicoletta.guerrieri GUERRIERI NICOLETTA |
