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DatoValore
TitleFast pesticide pre-screening in marine environment using a green microalgae-based optical bioassay
AbstractThe present study evaluates an optical bioassay based on green photosynthetic microalgae as a promising alternative for monitoring of relevant seawater pollutants. Photosystem II fluorescence parameters from several microalgae species were examined in the presence of three common marine pesticides that act as photosynthesis inhibitors. The three pollutants were detected within 10 min in concentrations between ng/L-mu g/L. The different algae species showed slightly diverse pesticide sensitivities, being Chlorella mirabilis the most sensitive one. Potential interferences due to oil-spill pollutants were discarded. The lipid content was characterized to identify microorganisms with suitable mechanisms that could facilitate stress acclimatization. C. mirabilis presented elevated content of unsaturated lipids, showing a promising potential for biosensing in saline stress conditions.
SourceMarine pollution bulletin. 129 (1), pp. 212–221
KeywordsBioassayPesticidePhotosynthesisMicroalgaChlorella mirabilis
JournalMarine pollution bulletin.
EditorMacmillan,, London, Regno Unito
Year2018
TypeArticolo in rivista
DOI10.1016/j.marpolbul.2018.02.036
AuthorsMoro, Laura; Pezzotti, Gianni; Turemis, Mehmet; Sanchis, Josep; Farre, Marinella; Denaro, Renata; Giacobbe, Maria Grazia; Crisafi, Francesca; Giardi, Maria Teresa
Text445709 2018 10.1016/j.marpolbul.2018.02.036 ISI Web of Science WOS 000433401600026 Bioassay Pesticide Photosynthesis Microalga Chlorella mirabilis Fast pesticide pre screening in marine environment using a green microalgae based optical bioassay Moro, Laura; Pezzotti, Gianni; Turemis, Mehmet; Sanchis, Josep; Farre, Marinella; Denaro, Renata; Giacobbe, Maria Grazia; Crisafi, Francesca; Giardi, Maria Teresa Biosensor Srl; Inst Environm Assessment Water Res IDAEA CSIC; Inst Coastal Marine Environm IAMC CNR; CNR Area Ric Roma The present study evaluates an optical bioassay based on green photosynthetic microalgae as a promising alternative for monitoring of relevant seawater pollutants. Photosystem II fluorescence parameters from several microalgae species were examined in the presence of three common marine pesticides that act as photosynthesis inhibitors. The three pollutants were detected within 10 min in concentrations between ng/L mu g/L. The different algae species showed slightly diverse pesticide sensitivities, being Chlorella mirabilis the most sensitive one. Potential interferences due to oil spill pollutants were discarded. The lipid content was characterized to identify microorganisms with suitable mechanisms that could facilitate stress acclimatization. C. mirabilis presented elevated content of unsaturated lipids, showing a promising potential for biosensing in saline stress conditions. The optimized microalgae based bioassay was preliminarily incorporated into a marine buoy for autonomous pre screening of pesticides in coastal areas, demonstrating its suitability for real time monitoring of marine water and quantitative evaluation of total biotoxicity. 129 Published version Fast pesticide pre screening in marine environment using a green microalgae based optical bioassay 1 s2.0 S0025326X18301243 main.pdf Articolo in rivista Macmillan, 0025 326X Marine pollution bulletin. Marine pollution bulletin. Mar. Pollut. Bull. Marine pollution bulletin. maria.giacobbe GIACOBBE MARIA renata.denaro DENARO RENATA francesca.crisafi CRISAFI FRANCESCA