Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Factors affecting the efficiency of molecular species delimitation in a species-rich insect family |
| Abstract | In the contest of global biodiversity loss, molecular species delimitation approaches can be very useful for accelerating species discovery through DNA taxonomy and inventory through DNA metabarcoding. In this study, the effect of some intrinsic factors on the efficiency of various single-marker species delimitation methods (fixed and variable nucleotide distance thresholds, ABGD, ASAP, GMYC, mPTP) was tested on more than 90 empirical datasets, derived from a set of 7,237 COI sequences attributed to 542 leaf beetles species (Coleoptera: Chrysomelidae). The considered factors were: i) the number of haplotypes per species (as a proxy for genetic diversity); ii) the geographic distance among conspecific collection localities (as a proxy of sampling width); iii) the difficulty related to morphological identification of species; iv) the taxonomic rank. Distance-based methods, with on average more than 70% of match with morphological identification, outperformed those relying on phylogenetic trees, with less than 59%. A high number of haplotypes per species was found to have a negative effect on delimitation efficiency, whereas large geographic distances within species had a positive effect. All methods delimitations (except for GMYC) were significantly affected by the presence of species that are difficult to be identified, decreasing their efficiency. Finally, the only method influenced by the taxonomic rank of the dataset was GMYC, showing lower efficiency in datasets at the genus than at higher levels. The observed biases we highlighted affecting efficiency could be accounted for when developing input datasets for species delimitation analyses to obtain a more reliable representation of biological diversity. |
| Source | Molecular ecology resources (Print) |
| Keywords | dna taxonomybeetles |
| Journal | Molecular ecology resources (Print) |
| Editor | Blackwell,, Oxford, Regno Unito |
| Year | 2021 |
| Type | Articolo in rivista |
| DOI | 10.1111/1755-0998.13352 |
| Authors | Giulia Magoga, Diego Fontaneto, Matteo Montagna |
| Text | 444608 2021 10.1111/1755 0998.13352 dna taxonomy beetles Factors affecting the efficiency of molecular species delimitation in a species rich insect family Giulia Magoga, Diego Fontaneto, Matteo Montagna Unimi, CNR IRSA In the contest of global biodiversity loss, molecular species delimitation approaches can be very useful for accelerating species discovery through DNA taxonomy and inventory through DNA metabarcoding. In this study, the effect of some intrinsic factors on the efficiency of various single marker species delimitation methods fixed and variable nucleotide distance thresholds, ABGD, ASAP, GMYC, mPTP was tested on more than 90 empirical datasets, derived from a set of 7,237 COI sequences attributed to 542 leaf beetles species Coleoptera Chrysomelidae . The considered factors were i the number of haplotypes per species as a proxy for genetic diversity ; ii the geographic distance among conspecific collection localities as a proxy of sampling width ; iii the difficulty related to morphological identification of species; iv the taxonomic rank. Distance based methods, with on average more than 70% of match with morphological identification, outperformed those relying on phylogenetic trees, with less than 59%. A high number of haplotypes per species was found to have a negative effect on delimitation efficiency, whereas large geographic distances within species had a positive effect. All methods delimitations except for GMYC were significantly affected by the presence of species that are difficult to be identified, decreasing their efficiency. Finally, the only method influenced by the taxonomic rank of the dataset was GMYC, showing lower efficiency in datasets at the genus than at higher levels. The observed biases we highlighted affecting efficiency could be accounted for when developing input datasets for species delimitation analyses to obtain a more reliable representation of biological diversity. Published version https //onlinelibrary.wiley.com/doi/10.1111/1755 0998.13352 mag 1755 0998.13352.pdf Articolo in rivista Blackwell, 1755 098X Molecular ecology resources Print Molecular ecology resources Print Mol. ecol. resour. Print Molecular ecology resources. Print diego.fontaneto FONTANETO DIEGO |