| Text | 444163 2021 10.1038/s41396 020 00887 6 Scopus 2 s2.0 85099978500 bioremediation dehalococcoides mccartyi sulfurospirillum Interspecies metabolite transfer and aggregate formation in a co culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene Kruse S.; Turkowsky D.; Birkigt J.; Matturro B.; Franke S.; Jehmlich N.; von Bergen M.; Westermann M.; Rossetti S.; Nijenhuis I.; Adrian L.; Diekert G.; Goris T. Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany, , Germany; Department Molecular Systems Biology, Helmholtz Centre for Environmental Research UFZ, Leipzig, Department Molecular Systems Biology, Helmholtz Centre for Environmental Research UFZ, Leipzig, Germany, , Germany; Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research UFZ, Leipzig, Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research UFZ, Leipzig, Germany, , Germany; Water Research Institute, IRSA CNR, Monterotondo, Rome, Water Research Institute, IRSA CNR, Monterotondo, Rome, Italy, , Italy; Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, Leipzig, Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, Leipzig, Germany, , Germany; Center for Electron Microscopy of the University Hospital Jena, Jena, Center for Electron Microscopy of the University Hospital Jena, Jena, Germany, , Germany; Chair of Geobiotechnology, Technische Universitat Berlin, Berlin, Chair of Geobiotechnology, Technische Universitat Berlin, Berlin, Germany, , Germany; Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research UFZ, Leipzig, Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research UFZ, Leipzig, Germany, , Germany; German Institute of Human Nutrition, Department Molecular Toxicology, Research Group Intestinal Microbiology, Potsdam Rehbrucke, Nuthetal, German Institute of Human Nutrition, Department Molecular Toxicology, Research Group Intestinal Microbiology, Potsdam Rehbrucke, Nuthetal, Germany, , Germany; Eurofins Institute Dr. Appelt Leipzig, Leipzig, Eurofins Institute Dr. Appelt Leipzig, Leipzig, Germany, , Germany Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular interactions between dehalogenating bacteria, we co cultured Sulfurospirillum multivorans, dechlorinating tetrachloroethene PCE to cis 1,2 dichloroethene cDCE , and Dehalococcoides mccartyi strains BTF08 or 195, dehalogenating PCE to ethene. The co cultures were cultivated with lactate as electron donor. In co cultures, the bacterial cells formed aggregates and D. mccartyi established an unusual, barrel like morphology. An extracellular matrix surrounding bacterial cells in the aggregates enhanced cell to cell contact. PCE was dehalogenated to ethene at least three times faster in the co culture. The dehalogenation was carried out via PceA of S. multivorans, and PteA a recently described PCE dehalogenase and VcrA of D. mccartyi BTF08, as supported by protein abundance. The co culture was not dependent on exogenous hydrogen and acetate, suggesting a syntrophic relationship in which the obligate hydrogen consumer D. mccartyi consumes hydrogen and acetate produced by S. multivorans. The cobamide cofactor of the reductive dehalogenase mandatory for D. mccartyi was also produced by S. multivorans. D. mccartyi strain 195 dechlorinated cDCE in the presence of norpseudo B produced by S. multivorans, but D. mccartyi strain BTF08 depended on an exogenous lower cobamide ligand. This observation is important for bioremediation, since cofactor supply in the environment might be a limiting factor for PCE dehalogenation to ethene, described for D. mccartyi exclusively. The findings from this co culture give new insights into aggregate formation and the physiology of D. mccartyi within a bacterial community. Published version http //www.scopus.com/record/display.url eid=2 s2.0 85099978500 origin=inward Articolo in rivista Nature Publishing Group, 1751 7362 The ISME journal Print The ISME journal Print ISME j. Print The ISME journal Print International Society for Microbial Ecology journal Print simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |
