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DatoValore
TitleMicroplastic-associated biofilms in lentic Italian ecosystems
AbstractIn this study, 16S rRNA gene high throughput sequencing and Fluorescence In Situ Hybridization (FISH) combined with confocal laser scanning microscopy (CLSM) were used to assess for the first time biodiversity and structure of microplastic-associated biofilms (plastisphere) collected from Italian lentic ecosystems. The analysis revealed clear differences in microbial community composition among biofilms and corresponding planktonic populations indicating a selective adhesion on microplastics (MP). Although geographical variations in taxa composition were observed, a plastisphere core microbiome, composed by known biofilm formers found in freshwater ecosystems (e.g. Sphingorhabdus, Sphingomonas, Rhodobacter, Aquabacterium and Acidovorax genera) was found. Species composition of plastisphere did not substantially differ between the diverse polymers, while a clear link with the MP exposure time was found by Fourier Transform Infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM) analysis. Generalist planktonic taxa (e.g. members of the families Sphingomonadaceae and Rhodobacteraceae) were found on MPs with the lowest degradation level whereas the biodiversity increased with the increase of MP degradation. FISH-CLSM analysis confirmed the Burkolderiaceae dominance in most of the analyzed plastisphere samples and revealed a patchy microbial colonization and a complex biofilm architecture with bacterial micro-colonies and cyanobacterial aggregates occurring together with microalgae assemblages.
SourceWater research (Oxf.) 187
KeywordsmicroplasticbiofilmPlastisphereBacterial biodiversity
JournalWater research (Oxf.)
EditorPergamon Press., New York, Regno Unito
Year2020
TypeArticolo in rivista
DOI10.1016/j.watres.2020.116429
AuthorsDi Pippo F.; Venezia C.; Sighicelli M.; Pietrelli L.; Di Vito S.; Nuglio S.; Rossetti S.
Text440160 2020 10.1016/j.watres.2020.116429 Scopus 2 s2.0 85091070396 microplastic biofilm Plastisphere Bacterial biodiversity Microplastic associated biofilms in lentic Italian ecosystems Di Pippo F.; Venezia C.; Sighicelli M.; Pietrelli L.; Di Vito S.; Nuglio S.; Rossetti S. Water Research Institute, CNR IRSA; Italian National Agency for New Technologies, Energy and Sustainable Economic Development ENEA ; LEGAMBIENTE In this study, 16S rRNA gene high throughput sequencing and Fluorescence In Situ Hybridization FISH combined with confocal laser scanning microscopy CLSM were used to assess for the first time biodiversity and structure of microplastic associated biofilms plastisphere collected from Italian lentic ecosystems. The analysis revealed clear differences in microbial community composition among biofilms and corresponding planktonic populations indicating a selective adhesion on microplastics MP . Although geographical variations in taxa composition were observed, a plastisphere core microbiome, composed by known biofilm formers found in freshwater ecosystems e.g. Sphingorhabdus, Sphingomonas, Rhodobacter, Aquabacterium and Acidovorax genera was found. Species composition of plastisphere did not substantially differ between the diverse polymers, while a clear link with the MP exposure time was found by Fourier Transform Infrared spectroscopy FT IR and Scanning Electron Microscopy SEM analysis. Generalist planktonic taxa e.g. members of the families Sphingomonadaceae and Rhodobacteraceae were found on MPs with the lowest degradation level whereas the biodiversity increased with the increase of MP degradation. FISH CLSM analysis confirmed the Burkolderiaceae dominance in most of the analyzed plastisphere samples and revealed a patchy microbial colonization and a complex biofilm architecture with bacterial micro colonies and cyanobacterial aggregates occurring together with microalgae assemblages. 187 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85091070396 origin=inward Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. simona.rossetti ROSSETTI SIMONA francesca.dipippo DI PIPPO FRANCESCA